目的 研究黄芪和当归不同剂量比例配伍对骨髓造血功能抑制模型小鼠造血祖细胞增殖能力和细胞衰老的影响，探讨黄芪和当归配伍促造血作用的机制。方法 ICR雄性小鼠，随机分为对照组、模型组、阳性药对照组、黄芪组、当归组、黄芪-当归不同剂量配伍组，分别ig给予不同配伍比例的黄芪-当归药液，每天1次，连续8 d。阳性对照组于给药的第6、7、8天sc重组人粒细胞集落刺激因子（rhG-CSF）。除对照组外，其余各组于给药的第4、5、6天ip环磷酰胺（CTX）造成骨髓造血功能抑制模型。于实验第9天处死动物取骨髓细胞进行红系、粒系、巨核系3系造血祖细胞培养，并以β-半乳糖苷酶染色法检测骨髓有核细胞衰老率。结果 与模型组比较，单用当归组、黄芪-当归（5：1）组、黄芪-当归（1：1）组、黄芪-当归（1：5）组均能显著升高粒细胞-单核细胞集落形成单位（CFU-GM）、巨核细胞集落形成单位（CFU-MK）、红细胞集落形成单位（CFU-E）、红系爆式集落形成单位（BFU-E）集落产率（P<0.01）；黄芪-当归（10：1）组可升高CFU-E、BFU-E、CFU-MK的集落产率（P<0.05、0.01），而对CFU-GM集落产率无显著影响。黄芪-当归（1：1）组促进造血祖细胞集落形成的作用显著强于单用黄芪、单用当归和其他比例配伍组（P<0.05）。与模型组比较，单用黄芪组、单用当归组、黄芪-当归（10：1）组、黄芪-当归（5：1）组、黄芪-当归（1：1）组、黄芪-当归（1：5）组骨髓有核细胞衰老阳性率显著降低（P<0.01），黄芪-当归（1：1）组骨髓有核细胞衰老阳性率显著低于单用黄芪、单用当归和其他比例配伍（P<0.05、0.01）。结论 黄芪、当归和黄芪-当归10：1、5：1、1：1、1：5配伍均可不同程度地促进骨髓造血功能抑制小鼠模型骨髓造血祖细胞增殖和分化，抑制骨髓造血细胞功能衰老，以黄芪-当归1：1配伍作用为优。提示促进骨髓造血祖细胞增殖和分化是黄芪和当归配伍促造血作用机制之一。

Objective To investigate the effects of different proportions of Astragalus and Angelica on the proliferation ability and cell senescence of hematopoietic progenitor cells (HPC) in the mice model of bone marrow hematopoiesis suppression, and to probe the mechanism of Astragalus-Angelica compatibility on promoting hematopoiesis. Methods ICR male mice were randomly divided into normal group, model group, positive control group of recombinant human granulocyte colony stimulating factor (rhG-CSF), Astragalus group, Angelica group, and different proportion combination groups of Astragalus and Angelica, and the animals in Chinese medicinal herb groups were ig administered, once a day, for 8 d. In the positive control group, rhG-CSF was sc injected on days 6, 7, and 8 of administration. Except for the normal group, the others were received cyclophosphamide (CTX) by ip injection on days 4, 5, and 6 of administration to establish the model of bone marrow hemopoiesis suppression. The mice were killed on day 9 to obtain bone marrow cells for the culture of HPC, and the senescence rate of bone marrow nucleated cell (BMNC) was detected by SA-β-galactosidase staining method. Results Compared with the model group, Angelica and Astragalus-Angelica with proportions of 5:1, 1:1, and 1:5 could significantly raise colony forming unit-granulocyte (CFU-GM), colony forming unit-megakaryocyte (CFU-MK), colony forming unit-erythroid (CFU-E), and burst forming unit-erythroid (BFU-E) (P<0.01). Astragalus-Angelica with 10:1 made CFU-MK, CFU-E, BFU-E remarkably increase (P<0.05, 0.01), and had no effect on CFU-GM. Furthermore, Astragalus-Angelica with 1:1 promoting the formation of HPC was evidently stronger than that of single Astragalus, single Angelica, and other combinations (P<0.05). Compared with the model group, the senescence positive rate of BMNC was markedly decreased in Astragalus group, Angelica group, and Astragalus-Angelica combination groups (P<0.01), while being lowest in Astragalus-Angelica with 1:1 proportion (P<0.05, 0.01). Conclusion Astragalus, Angelica, Astragalus-Angelica with 10:1, 5:1, 1:1, and 1:5 proportions can prompt the proliferation and differentiation of HPC that bone marrow hematopoiesis is suppressed in mice, inhibit the senescence of bone marrow hematopoietic cell, moreover, the effect of Astragalus-Angelica with 1:1 proportion is the best. It suggests that promoting the proliferation and differentiation of HPC is one of the mechanisms that Astragalus-Angelica prompt hematopoiesis.

国家自然科学基金资助项目（81473581）；“中西医结合防治心脑血管疾病的相关基础研究”湖南省高校科技创新团队；“中医药防治心脑血管疾病基础研究”湖南省自然科学创新群体基金