[关键词]
[摘要]
目的 进一步评价丹参酮IIA纳米结构脂质载体(TIIA-NLC)的性质,并考察其对HaCaT细胞增殖的影响。方法 采用纳米粒度仪及HPLC法对TIIA-NLC的粒径和光稳定性进行考察,透析袋法测定TIIA-NLC在72 h内的累积释放量并绘制释放曲线,MTT法考察TIIA-NLC对HaCaT细胞增殖的影响。结果 TIIA-NLC的平均粒径为(178±9)nm,多分散度指数(PDI)为0.183±0.017,Zeta电位(-27.5±5.6)mV,体外72 h累积释放量为52.28%,TIIA-NLC能显著降低TIIA的光降解速率,TIIA在一定范围内以剂量依赖性的方式抑制HaCaT细胞增殖,同质量浓度的TIIA-NLC对HaCaT细胞增殖的抑制作用显著高于TIIA溶液。结论 TIIA-NLC稳定性好,具有良好的缓释作用,较好的细胞生物相容性,能显著提高TIIA对HaCaT细胞增殖的抑制作用。
[Key word]
[Abstract]
Objective To evaluate TIIA-NLC's quality in vitro and study its effects on the HaCaT cells. Methods Its particle size, polydispersity index (PDI) and photostability were investigated by Zetasizer analyzer and high-performance liquid chromatography (HPLC). The in vitro release of TIIA-NLC within 72 h was measured by dialysis method; Meanwhile MTT assay was used to detect proliferation activity on HaCaT cells. Results The average particle size, PDI and Zeta potential of TIIA-NLC was:(178±9) nm, 0.183±0.017 and (-27.5±5.6) mV. The 72 h in vitro accumulative release rate was 52.28%, and the degradation velocity of TIIA was significantly slowed down. The TIIA-NLC inhibited the proliferation of HaCaT cells in concentration-dependent mode within a certain range. Compared with TIIA group, TIIA-NLC had more effects on the HaCaT cells. Conclusion The prepared TIIA-NLC showed good stability, sustained release of interest, good cell biology intermiscibility, and could obviously increase the effects on the HaCaT cells.
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[基金项目]
国家自然科学基金资助项目(81573696)