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[摘要]
目的 建立同时测定红景天中ternatumoside II、红景天欧素、红景天宁、草质素、山柰酚、大花红景天素的方法,比较不同产地红景天中6个成分的差异。方法 采用HPLC-DAD法,Kromasil C18(250 mm×4.6 mm,5 μm)色谱柱,乙腈-水为流动相,梯度洗脱,体积流量为1.0 mL/min,检测波长为382 nm,柱温为35℃。结果 ternatumoside II、红景天欧素、红景天宁、草质素、山柰酚、大花红景天素进样量分别在0.015 08~0.301 6、0.123 2~2.464、0.109 5~2.190 8、0.007 8~0.156、0.021 46~0.429 2、0.006 48~0.129 6 μg与色谱峰面积呈良好的线性关系;加样回收率在98.17%~100.52%,RSD在1.64%~1.98%。结论 该方法简单准确,为红景天的全面质量控制提供了参考。
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[Abstract]
Objective To establish a determination method for the contents of ternatumoside II,rhodiosin,rhodionin,herbacetin,kaempferol,and rhodiolin in Rhodiolae Crenulatae Radix et Rhizoma and to compare the content differences of the six flavonid compounds in Rhodiolae Crenulatae Radix et Rhizoma from different sources.Methods Using HPLC-DAD method and Kromasil C18 column (250 mm×4.6 mm,5 μm),with acetonitrile and water as mobile phase,gradient elution at flow rate of 1.0 mL/min and column temperature of 35℃.The detection wavelength was 382 nm.Results Ternatumoside II,rhodiosin,rhodionin,herbacetin,kaempferol,and rhodiolin had good linearity in the ranges of 0.015 08-0.301 6,0.123 2-2.464,0.109 5-2.190 8,0.007 8-0.156,0.021 46-0.429 2,and 0.006 48-0.129 6 μg,respectively.The average recoveries of the six constituents were 98.17%-100.52% and RSD values were 1.64%-1.98%.Conclusion The method is simple and accutate.It provides the reference for comprehensive quality control of Rhodiolae Crenulatae Radix et Rhizoma.
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