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[摘要]
目的 考察颠茄草不同生长期8种化合物的动态变化,探索各成分在不同部位的分布。方法 采用反相高效液相色谱法-二极管阵列检测器,以乙腈-0.05%磷酸溶液为流动相梯度洗脱,同时测定东莨菪苷、绿原酸、槲皮素-3-O-半乳糖-(6→1)鼠李糖-7-O-葡萄糖苷、槲皮素-3-O-葡萄糖-(6→1)鼠李糖-7-O-葡萄糖苷、山柰素-3-O-半乳糖-(6→1)鼠李糖-7-O-葡萄糖苷、山柰素-3-O-葡萄糖-(6→1)鼠李糖-7-O-葡萄糖苷、东莨菪内酯和芦丁的量,并比较各成分在不同生长期的变化和在不同部位的分布。结果 各成分在整个植株中的总量随植株的生长呈上升趋势,但不同成分增长速率不同,大部分成分在6月中下旬达到平衡;东莨菪苷主要分布在根部,绿原酸以叶和花中量较高,东莨菪内酯各部位分布较均匀,其他5种成分在叶和花中量较高,根部几乎不含。结论 颠茄草中各成分生长速率不同,且各部位分布不同,各成分量存在较大差异。
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[Abstract]
Objective To investigate the dynamic accumulations and distributions of eight chemical compounds of Belladonnae Herba in different growing periods and explore the distribution of each ingredient in various parts. Methods The detection was performed by RP-HPLC with diode array detector (DAD). The mobile phase was acetonitrile-0.05% phosphonic acid in gradient elution. The HPLC method was established for the simultaneous determination of the eight compounds, such as scopolin, chlorogenic acid, quercetin-3-O-galactose (6→1) rhamnose-7-O-glucoside, quercetin-3-O-glucose (6→1) rhamnose-7-O-glucoside, kaempferol-3-O-galactose (6→1) rhamnose-7-O-glucoside, kaempferol-3-O-glucose-(6→1) rhamnose-7-O-glucoside, scopoletin, and rutin, and also for the the comparison on the changes of each ingredient in different growing periods and distribution in various parts. Results The total amount of each ingredient in the whole plant increased along with its growth. However, each ingredient had different rate of increase, in addition, most compounds would reach to a balance of accumulation in the middle or the last third of June. The analysis results showed scopolin mainly distributed in the roots, while chlorogenic acid had a higher distribution in the leaves and flowers; Scopoletin distributed evenly among all parts in the plant, and the others possessed a higher distribution among the leaves and flowers, however, there was scare distribution in the roots. Conclusion Each ingredient of Belladonnae Herba has different rate of increase and different distribution in each part, resulting in comparatively large difference of each compound. The experimental data of this research could provide the basis for the implantation, harvest and collection, and quality evaluation of Belladonnae Herba.
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