目的 探讨SIRT1/NF-κB信号轴在人参皂苷Rg₁对抗衰老模型大鼠造血干/祖细胞衰老中的作用。方法 6~8周雄性SD大鼠50只，随机分为对照组、模型组、人参皂苷Rg₁对照组、人参皂苷Rg₁治疗组和人参皂苷Rg₁预防组，每组10只。以连续42 d sc D-半乳糖（D-gal）制备衰老动物模型，人参皂苷Rg₁各组ip给药不同时间，药物注射完成第2天，免疫磁性分选法分离纯化各组Sca-1⁺造血干/祖细胞（HSC/HPC），衰老相关β-半乳糖苷酶（SA-β-Gal）染色、流式细胞周期分析和造血祖细胞混合集落（CFU-Mix）培养观察人参皂苷Rg₁对Sca-1⁺ HSC/HPC抗衰老的生物学作用。荧光定量PCR及Western blotting检测衰老调控分子SIRT1、NF-κB mRNA及蛋白的表达。结果 与模型组比较，人参皂苷Rg₁治疗组和预防组SA-β-Gal染色阳性率、G₀/G₁期细胞比例下降，生成CFU-Mix数增高，SIRT1 mRNA及蛋白表达上调，NF-κB mRNA及蛋白表达下调；人参皂苷Rg₁预防组各指标变化均较人参皂苷Rg₁治疗组明显。结论 SIRT1/NF-κB信号轴在人参皂苷Rg₁对抗D-gal致衰老模型大鼠HSC/HPC衰老过程中发挥重要作用，人参皂苷Rg₁具有抗HSC/HPC衰老作用。

Objective To investigate the effect of SIRT1/NF-κB signal axis on delaying hematopoietic stem cell and progenitor cell senescence with ginsenoside Rg₁ in ageing model rat induced by D-galactose. Methods Male SD rats (n=40) aging from 6 to 8 weeks old were randomly divided into control group, aging model group, positive control group, Rg₁ treated group, and Rg₁ prevented group (n=10). The aging rat model was prepared by sc D-galactose for continuous 42 d, then ginsenoside Rg₁ was given in different time. After 2 d of the treatment, the Sca-1⁺ HSC/HPC was isolated by magnetic cell sorting (MACS). The changes of cells observed by senescence-associated β-galactosidase (SA-β-Gal) staining, cell cycle analysis and culture of mixed hematopoietic progenitor cell were used to investigate the treated aging effect of ginsenoside Rg₁.The expression of senescence associated SIRT1, NF-κB mRNA and protein was examined by real time fluorescence quantitative PCR (FQ-PCR) and Western blotting. Results In ginsenoside Rg₁ treated group and ginsenoside Rg₁ prevented group, the percentage of positive cells expressed SA-β-Gal and the number of cells entering G₀/G₁ phase were lower than that of aging model group, but the number of CFU-Mix was increased than aging model group. Compared with aging model group, the expression of SIRT1 mRNA and protein was upregulated and the expression of NF-κB mRNA and protein was downregulated in Rg₁ treated group and prevented group. Changes in Rg₁ prevented group were more than those in Rg₁ treated group. Conclusion SIRT1/NF-κB signal axis may play a key role in the anti-aging effect of Rg₁ to Sca-1⁺ HSC/HPC senescence in ageing model rat induced by D-galactose.

国家自然科学基金资助项目（81660731，81673748，81202785）；云南省教育厅科学研究基金资助性项目（2016YJS118）