目的 通过橙皮苷的临床利用量对青皮的品质进行评价研究。方法 在单因素试验基础上，以橙皮苷提取转移率和浸膏得率的总评归一值（OD）为因变量，加水量、提取时间为影响因素，利用星点设计2因素5水平试验，进行2次多项式拟合，通过效应面法优选青皮提取工艺。并根据最佳提取工艺，在“真实量”以及提取转移率基础上，测定了市场上11批青皮中橙皮苷的临床利用量。结果 青皮的最佳提取工艺为加24倍量水，提取3次，每次2.5 h，OD预测值为0.822 0，验证值为0.808 8，与预测值偏差为1.61%。11批青皮中橙皮苷的临床利用量大小与其量高低并非正相关。结论 建立的方法将药材中的有效成分与临床实际利用情况相联系，为中药材品质评价研究提供了一种新的思路。

Objective To evaluate the quality of Citri Reticulatae Pericarpium Viride (CRPV) through the clinically utilized quantity of hesperidin. Methods On the basis of single-factor experiments, extraction technology of CRPV was optimized by solvent volume and extraction time as independent variables, overall desirability value of extract yield and the extraction transfer rate of hesperidin as evaluation indexes. Central composite design was adopted to design a two factors-five levels table, and a quadratic-multinomial fitting was applied. Extraction process was selected by response surface methodology. According to the optimum extraction process, the clinically utilized quantity of 11 batches of CRPV was determined based on the real content and extraction transfer rate. Results Optimal extraction technology was as follows:extracted for three times with 24-folds of water, 2.5 h each time. The model-predicted and experimentally measured values of overall desirability were 0.822 0 and 0.808 8, respectively, revealing a relative error of only 1.61% between them. The clinically utilized quantity and the contents of hesperidin of 11 batches of CRPV was not positive correlation. Conclusion The method established in this paper is related to the effective components of the medicinal materials and the clinical practice, which provides a new way of thinking for the quality evaluation of Chinese medicinal materials.

四川省千人计划支持项目（2013332号）