目的 建立基于一测多评法（QAMS）同时测定大枣药材中多种三萜酸类成分的分析方法，为大枣药材的品质评价和质量控制提供技术支撑。方法 采用HPLC-ELSD技术，以白桦脂酸对照品为内参物，测定其与齐墩果酸、熊果酸、桦木酮酸、齐墩果酮酸、熊果酮酸的相对校正因子，采用相对校正因子计算此5种三萜酸类化学成分的量，实现一测多评；同时比较外标法所测得的大枣药材中该6种化学成分的实际值与经QAMS所得的计算值之间的差异，以验证QAMS的适用性、可行性和重复性。结果 相对校正因子的重现性较好，15批大枣药材样品中6种三萜酸类化学成分的QAMS计算值与外标法实测值之间无显著性差异。结论 以白桦脂酸为内参物建立的同时测定齐墩果酸、熊果酸、桦木酮酸、齐墩果酮酸、熊果酮酸的一测多评方法操作简便，测定结果准确，实现了采用1种对照品同步测定大枣样品中6种化学成分的量，可为大枣药材质量标准的制定和提升提供支撑，为中药多指标成分质量评价模式的建立提供借鉴。

Objective To develop a method for the simultaneous determination of several triterpenic acids in Jujubae Fructus and provide the technical support for the quality control of Jujubae Fructus.Methods Betulinic acid was used as the internal reference substance, and the relative correlation factors (RCF) of oleanolic acid, ursolic acid, betulonic acid, oleanonic acid, and ursonic acid were determined by HPLC-ELSD. The contents of the five components were calculated according to the RCF, respectively. The contents of these six triterpene acids in 15 batches of Jujubae Fructus were determined by the external standard method. The rationality, feasibility, and repeatability for the method of quantitative analysis of multi-components by single-marker (QAMS) were verified by comparing with the results obtained from the external standard method.Results The reproducibility of the RCF was acceptable. For the six triterpene acids, there was no significant difference between the quantitative results with the two different methods in the 15 batches of Jujubae Fructus.Conclusion The method established in this research is accurate and feasible that it just needs to assay single-marker (betulinic acid) for the determination of six triterpenic acids in Jujubae Fructus simultaneously. Therefore, this method is helpful for the quality control of Jujubae Fructus and could provide a new reference for the quality assess of multi components in Chinese materia medica.

国家自然科学基金项目（81473538）；教育部霍英东教育基金会高等院校青年教师基金项目（141040）；江苏高校优势学科建设工程资助项目（ysxk-2014）