目的 建立HPLC法同时测定葎草中木犀草苷、芹菜素-7-O-β-D-葡萄糖苷、木犀草素量，并测定不同产地、不同采收月份、雌株和雄株葎草中木犀草苷、芹菜素-7-O-β-D-葡萄糖苷、木犀草素的量，为葎草质量标准研究提供科学依据。方法 HPLC法同时测定葎草中木犀草苷、芹菜素-7-O-β-D-葡萄糖苷、木犀草素的量。系统适用性条件，色谱柱为Agilent 5 TC-C₁₈（250 mm×4.6 mm，5 μm）；流动相为乙腈-0.2%磷酸水溶液，梯度洗脱；体积流量1.0 mL/min；检测波长340 nm；柱温25℃；进样量10 μL。结果 木犀草苷、芹菜素-7-O-β-D-葡萄糖苷、木犀草素的检测质量浓度分别在4.640～74.24、1.510～30.20、0.796 3～12.74 μg/mL与各自峰面积值呈良好线性关系（r=0.999 2、0.999 9、0.999 5），精密度、稳定性、重复性试验的RSD均≤1.99%，平均回收率分别为102.69%、100.49%、100.39%，RSD分别为1.64%、1.93%、1.65%（n=9）。结论 在河北省不同产地、不同采收月份、雌株、雄株葎草药材中，木犀草苷、芹菜素-7-O-β-D-葡萄糖苷、木犀草素的量存在较大差异。

Objective To establish a method for the determination of luteoloside, apigenin-7-O-β-D-glucosidase, and luteolin by high performance liquid chromatography (HPLC) method, and the determination of luteoloside, apigenin-7-O-β-D-glucosidase, and luteolin in Humulus scandens female and male plants from different origins and different harvest months in Hebei province. Methods HPLC method was used for simultaneous determination of luteoloside, apigenin-7-O-β-D-glucosidase, and luteolin. System suitability conditions were as follows: column: Agilent 5 TC-C₁₈ (250 mm×4.6 mm, 5 μm); mobile phase: acetonitrile-0.2% phosphoric acid; flow rate: 1.0 mL/min; detection wavelength: 340 nm; column temperature: 25℃; injection volume: 10 μL. Results The linear ranges were 4.640-74.24 μg/mL of luteoloside (r=0.999 2), 1.510-30.20 μg/mL of apigenin-7-O-β-D-glucosidase (r=0.999 9), and 0.796 3-12.74 μg/mL of luteolin. RSD of precision, stability, and repeatabillity tests were≤1.99%. The average recoveries were 102.69% (RSD=1.64%, n=9), 100.49% (RSD=1.93%, n=9), and 100.39% (RSD=1.65%, n=9). Conclusion The method is simple, reproducible, and accurate, and reliable measurement results can be used for simultaneous determination of luteoloside, apigenin-7-O-β-D-glucosidase, and luteolin in H. scandens female and male plants from different origins and different harvest months in Hebei province. There is greater difference between luteoloside, apigenin-7-O-β-D-glucosidase, and luteolin in H. scandens female and male plants from different origins and different harvest months in Hebei province.