[关键词]
[摘要]
目的 通过化学组分测定和特征图谱分析,评价不同干燥方式对麦冬品质的影响。方法 总皂苷、总黄酮和总多糖量采用紫外分光光度法进行测定,麦冬皂苷D和麦冬甲基黄烷酮A的量采用HPLC法进行测定;特征图谱采用HPLC法建立。结果 不同干燥方法对麦冬化学组分量的影响差异显著,变异系数在6.9%~20.8%,其中,阴干、晒干、冷藏干燥和晒半干后烘干对麦冬化学组分破坏程度均较小,远红外干燥和微波干燥对麦冬化学组分破坏最大;麦冬样品有18个HPLC特征峰,聚类分析可将14种干燥方法分为3类,聚为一类的干燥方法干燥条件相近。结论 通过化学组分量变化和HPLC图谱特征能够有效地反映干燥方法的差异,可作为麦冬产地干燥方法筛选的技术指标,在麦冬产地加工过程中建议晒干和空气源热泵烘干机烘干相结合应用。
[Key word]
[Abstract]
Objective Using chemical component content determination and fingerprint analysis to evaluate the effects of different drying methods on quality of Ophiopogonis Radix. Methods The contents of total saponin, total flavonoids, and total polysaccharide were determined by ultraviolet spectrophotometry, and ophiopogonin D and methylophiopogonanone A were determined using HPLC; the chromatograms fingerprint was established by HPLC. Results The influence of different drying methods on the chemical components in Ophiopogonis Radixa was significantly different, and the coefficient of variation fell in the range of 6.9%-20.8%. Among them, the damage degree of dried in the shade, dried in the sun, freeze drying and dried after semi-died in the sun is small on chemical component of Ophiopogon japonicus. However, the far infrared drying and microwave drying have the most serious effect; Ophiopogonis Radix samples have 18 characteristic peaks through HPLC. The 14 kinds of drying methods were divided into three types by cluster analysis, and a class of drying methods has similar drying conditions. Conclusion The difference of drying method can be effectively reflected by the change of the content of chemical compositions and the feature of HPLC spectrum, which can be used as indicators of O. japonicus origin screening method. In the producing area, it is best to combine drying in the sun with in air source heat pump dryer in processing of Ophiopogonis Radix.
[中图分类号]
[基金项目]
川产道地药材麦冬、半夏的品种和质量评价研究(2014F038);四川三台县涪城麦冬集成技术推广与应用科技惠民项目(2013GS510102);中药质量安全检验检测和风险控制技术平台(2014ZX09304307-002);麦冬整合式产业链综合开发研究