目的 通过构建羟基桂皮酰辅酶A羟基桂皮酰转移酶（hydroxycinnamoyl CoA quinate hydrocycinnamoyl transferase，HQT）基因真核表达载体以及建立金银花的遗传转化系统，阐明金银花HQT基因在真核生物细胞中对绿原酸量的调控机制。方法 应用通路克隆系统技术构建HQT基因真核表达载体，利用根癌农杆菌介导法，构建含金银花HQT基因的工程菌，对转染成功的外植体进行愈伤诱导，进而进行半定量RT-PCR检测转基因愈伤组织中HQT基因的相对表达量以及利用HPLC检测其绿原酸的量，并对二者相关性进行分析。结果 金银花愈伤组织中绿原酸的量随HQT基因表达量的升高而升高。结论 金银花HQT基因在真核生物细胞内对绿原酸的生物合成具备调控作用。

Objective To clarify how the HQT gene regulates and controls the chlorogenic acid in eukaryotic cells of Lonicerae Flos by establishment of HQT gene eukaryotic expression system, construction and optimization of genetically modified tissue culture system. Methods Using gateway cloning system to build HQT gene eukaryotic expression system, we genetically created engineered bacteria containing honeysuckle gene function HQT by Agrobacterium tumefaciens-mediated method. The successful transfection explants of callus induction were established and the HQT gene relative transcript level was amplified by semi-quantitative RT-PCR; The HPLC was used to determine the sample's content of chlorogenic acid, then the correlation between HQT gene polymorphism and content of chlorogenic acid was analyzed. Results The content of chlorogenic acid in Lonicerae Flos callus quantity rises as its HQT gene expression. Conclusion Honeysuckle HQT gene could regulate chlorogenic acid biosynthesis in eukaryotic cells.

国家自然科学基金资助项目（31370360）