[关键词]
[摘要]
目的 采用高效液相色谱串联四级杆飞行时间质谱技术(HPLC-Q-TOF-MS/MS)对银黄清肺胶囊的化学成分进行检测和分析。方法 采用Inertsil ODS-2 C18色谱柱(250 mm×4.6 mm,5 μm)反相HPLC梯度洗脱法分离各主要成分,电喷雾电离源经正离子模式对色谱流出物进行检测,四级杆飞行时间串联质谱法对各主要色谱峰进行归属;采用对照品、检索文献及各成分的图谱数据分析的方法分析化学成分。结果 依据质谱裂解规律及文献检索,在正离子模式下,从银黄清肺胶囊样品中共鉴定出54个化合物。结论 本实验建立了一种基于HPLC-Q-TOF-MS/MS技术对复方银黄清肺胶囊中化学成分进行鉴别的有效方法,为其质量控制及物质基础研究提供了技术支持。
[Key word]
[Abstract]
Objective To identify and analyze the chemical constituents in Yinhuang Qingfei Capsule by HPLC-Q-TOF-MS/MS method. Methods The HPLC method was used with the conditions that the column was Inertsil ODS-2 C18 (250 mm×4.6 mm, 5 μm). Columu and electrospray ion (ESI) source was employed for the qualitative analysis under positive ion mode. These components were further analyzed by MS spectra, and by comparing with the corresponding reference substances and literature data. Results According to the MS principle and literature data, 54 compounds were identified from the sample of Yinhuang Qingfei Capsule. Conclusion An efficient HPLC-Q-TOF-MS/MS approach has been established for studying the chemical constituents in Yinhuang Qingfei Capsule, which paves a way for the quality control and further substance basis studies of the preparation.
[中图分类号]
[基金项目]
长沙市生物与新医药产业科技支撑重大专项项目:中药创新关键技术研究及产业化(K1204035-31)