目的 建立HPLC法同时测定桂枝茯苓胶囊（GFC）中4种茯苓三萜酸成分（去氢土莫酸、猪苓酸C、3-表去氢茯苓酸、去氢茯苓酸）的方法。方法 色谱柱为Diamonsic C₁₈柱（250 mm×4.6 mm，5 μm），流动相为乙腈-0.2%甲酸水溶液；梯度洗脱：0～70 min，50%～85%乙腈；70～80 min，85%～100%乙腈；80～90 min，100%乙腈；检测波长242 nm，体积流量1.0 mL/min，柱温40℃。结果 去氢土莫酸、猪苓酸C、3-表去氢茯苓酸、去氢茯苓酸在HPLC中达到基线分离，线性范围分别为0.408～2.04（r=0.999 9）、0.192～0.96（r=0.999 5）、0.078～0.39（r=0.999 5）、0.075 6～0.378（r=0.999 5）μg/mL；平均加样回收率分别为97.5%、98.5%、97.2%、102.3%，RSD分别为1.4%、1.6%、1.2%、1.8%；测定了6批GFC，结果显示去氢土莫酸平均量为0.070 mg/粒，猪苓酸C平均量为0.015 mg/粒，3-表去氢茯苓酸平均量为0.030 mg/粒，去氢茯苓酸平均量为0.061 mg/粒。结论 该方法可行、重现性好，可用于GFC的质量控制。

Objective To establish an HPLC method for the determination of four triterpene constituents(dehydrotumulosic acid,polyporenic acid C,3-epi-dehydropachymic acid,and dehydropachymic acid) in Guizhi Fuling Capsules(GFC).Methods Chromatography conditions were Diamonsic C₁₈ column(250 mm×4.6 mm,5 μm),system mobile phase was composed of acetonitrile(A)-0.2% HCOOH aqueous solution(C) in a linear gradient elution mode(0-70 min:50%-85% A;70-80 min:85%- 100% A;80-90 min:100% A),detective wavelength was set at 242 nm,and column temperature was 40℃.Results The calibration curve was linear within 0.408-2.04 μg/mL(r=0.999 9),0.192-0.96 μg/mL(r=0.999 5),0.078-0.39 μg/mL(r=0.999 5),and 0.075 6-0.378 μg/mL(r=0.999 5) for dehydrotumulosic acid,polyporenic acid C,3-epi-dehydropachymic acid,and dehydropachymic acid,respectively.The average recoveries were 97.5%(RSD=1.4%,n=5),98.5%(RSD=1.6%,n=5),97.2%(RSD=1.2%,n=5),and 102.3%(RSD=1.8%,n=5).Six batches of GFC sample were determined,The average contents of dehydrotumulosic acid,polyporenic acid C,3-epi-dehydropachymic acid,and dehydropachymic acid were 0.070,0.015,0.030,and 0.061 mg/capsule,separately.Conclusion The method is simple,accurate,and can be used as a quality control method for GFC.

国家“十二五”科技重大专项（2012ZX09103201-043，2012ZX09301002-002）