[关键词]
[摘要]
目的 建立白芷药材-饮片-提取物及配方颗粒的HPLC特征图谱,对其相关性进行评价。方法 采用HPLC法,色谱柱为Agilent HC-C18柱(250 mm×4.6 mm,5 μm);以乙腈-水为流动相梯度洗脱,检测波长320 nm,体积流量1.0 mL/min,柱温:25℃。以特征图谱为对照,对药材-饮片-提取物及配方颗粒进行相关性评价。结果 白芷10批药材、饮片、提取物及配方颗粒特征图谱均呈现10个共有特征峰,呈良好的相关性。结论 建立了白芷药材-饮片-提取物及配方颗粒的HPLC特征图谱,反映了白芷药材-饮片-提取物及配方颗粒多成分的整体面貌,为白芷配方颗粒质量控制提供借鉴。
[Key word]
[Abstract]
Objective To establish an HPLC characteristic spectrum of raw herbs-decoction pieces-extract-dispensing granules of Angelicae Dahuricae Radix(ADR) and evaluate the correlation among them.Methods Gradient elution was performed with mobile phase of acetonitrile-water,detection wavelength of 320 nm,flow rate of 1.0 mL/min,and column temperature of 25℃,by setting the characteristic spectrum as control and evaluating the correlation of raw herbs-decoction pieces-extract-dispensing granules.Results There were 10 common characteristic peaks in all characteristic spectra of 10 batches of raw herbs,decoction pieces,extract,and dispensing granules of ADR,presenting a good correlation.Conclusion This study established an HPLC characteristic spectrum and reflected the whole picture of multi-component of raw herbs,decoction pieces,extract,and dispensing granules of ADR provides the reference for quality control of dispensing granules of ADR.
[中图分类号]
[基金项目]
国家中医药管理局中医药科学技术研究专项(2013ZX02)