目的 建立定量测定多种丹参酚酸的高效液相色谱（HPLC）分析方法，考察丹参素和原儿茶醛对丹酚酸B降解规律的影响。方法 应用HPLC技术对丹酚酸B降解样品中的4种酚酸进行定量分析，应用LC-MS技术剖析样品的化学物质基础，对加入丹参素和原儿茶醛前后的丹酚酸B样品进行研究。结果 丹酚酸B降解产物主要有9种，包括丹参素、原儿茶醛、丹酚酸B的多种异构体、紫草酸以及多种紫草酸异构体。加入相同质量浓度的丹参素和原儿茶醛，原儿茶醛对丹酚酸B降解的保护作用更明显，紫草酸异构体Ⅰ和Ⅱ的峰面积随着初始原儿茶醛质量浓度的增大而有所增加。结论 原儿茶醛对丹酚酸B降解的保护作用可能是依赖于其弱酸性，紫草酸异构体Ⅰ和Ⅱ的峰面积受原儿茶醛初始质量浓度的影响可能与反应平衡改变有关。

Objective To develop a method for the quantitative determination of several salvianolic acids by HPLC and investigate the influence of danshensu and protocatechuic aldehyde on the degradation process of salvianolic acid B(SaB).Methods HPLC method was applied to quantitative analysis on four components in the degradation sample of SaB.HPLC-MS method was applied to tentatively characterizing the structure of the degradation products.The effect of danshensu and protocatechuic aldehyde on the degradation of SaB were found out by the analysis of chromatogram and mass spectra of degradation samples.Results Nine degradation products were found in the degradation samples of SaB,including danshensu,protocatechuic aldehyde,isomers of SaB,lithospermic acid,and its isomers.Protocatechuic aldehyde had stronger protective effect on SaB compared to danshensu at the same mass concentration.Additionally,the peak area of lithospermic acid isomers Ⅰ and Ⅱ increased when the initial concentration of protocatechuic aldehyde was increased.Conclusion The mild acidity of protocatechuic aldehyde might account for its stronger protective effect on SaB,and the connection between protocatechuic aldehyde and lithospermic acid isomers Ⅰ and Ⅱ might relate to the change of reaction balance.

国家自然科学基金资助项目（81273992）