目的 研究积雪草Centella asiatica皂苷水解产物的化学成分，以期发现新的三萜皂苷元。方法 通过硅胶、凝胶柱色谱对积雪草皂苷酸水解产物进行分离，经核磁共振、质谱、红外光谱、紫外光谱等波谱手段对化合物的结构进行鉴定。并对部分化合物的体外抗肿瘤活性进行了测试。结果从积雪草皂苷酸水解产物中分离得到4个单体苷元化合物，鉴定为2α,3β,23-三羟基乌苏-6,12-二烯-28-酸（1）、积雪草酸（2）、6β-羟基积雪草酸（3）、terminolic acid（4）。结论 化合物1为1个新的乌苏烷型苷元，命名为积雪草-6-烯，并且其对所测试的肿瘤细胞HL-60、A549、U873无抑制活性。

Objective To study the chemical constituents of the acid hydrolysis products,in order to find some new aglycones from the hydrolysate of the saponin of Centella asiatica.Methods The acid hydrolytic product was separated by repeated silica gel,Sephadex LH-20 chromatography.The cytotoxic activities in vitro of some compounds isolated were evaluated.Their structures isolated were identified by analysis of their spectral data of NMR,MS,IR,and UV.Results Four aglycones,2α,3β,23-trihydroxyurs-6,12-dien-28-oic acid(1),asiatic acid(2),6β-hydroxy asiatic acid(3),terminolic acid(4),were isolated from the hydrolysis product.Conclusions Compound 1,named centella-6-ene,was a new ursane-type aglycone and it showed no cytotoxic activities against the tested cancer cell lines.

国家自然科学基金面上项目（81273389）