[关键词]
[摘要]
目的 以洋常春藤Hedera helix 1年生扦插苗为材料,通过检测不同部位及1年生长周期内4种常春藤皂苷类化合物(α-常春藤皂苷、常春藤皂苷C、常春藤皂苷B和常春藤皂苷D)的量,分析洋常春藤皂苷类化合物代谢累积规律,以期为确定药用洋常春藤原料的合理采收时间提供理论依据。方法 实验通过合理设计洋常春藤材料的采样方法,采用HPLC法测定常春藤皂苷类化合物量。结果 洋常春藤不同部位皂苷类化合物量测定结果为老叶> 新叶> 茎> 根,并且在一年的生长周期内,不同常春藤皂苷类化合物在不同采样部位中的动态累积规律均有所不同。结论 洋常春藤叶片和茎均可作为高质量药用原料的采收部位,且叶片的最适宜采收时期为3月份开始的生长发育期,而茎的适宜采收时期则可以从3月份开始的生长发育期持续到低温休眠开始前的11月份。
[Key word]
[Abstract]
Objective This study detected the contents of four saponins (hederacoside C, hederacoside D, hederacoside D, α-hederin) from different parts of annual cutting Hedera helix in one year growth cycle and analyzed the dynamic accumulation, in order to provide theoretical basis to harvest Hedera helix L. as medicinal raw materials reasonably. Methods This study designed a reasonable method of collecting materials and detected the contents of saponins by HPLC. Results The contents of saponins in different parts were as following order: mature leaf > new leaf > stem > root, and four saponins in different parts had different dynamic accumulations in one year growth cycle. Conclusion The leaf and stem are the reasonable collecting parts of raw materials which have higher medicinal quality. The reasonable collecting time of leaf is the growth stage in March and stem is from March to November before the beginning of dormancy.
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[基金项目]
湖南省教育厅科研项目(15A089);湖南省研究生科研创新项目(CX2014B290)