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[摘要]
目的 采用多波长HPLC法建立仙灵骨葆胶囊中川续断皂苷VI、补骨脂素及异补骨脂素的定量测定方法。方法 采用月旭Ultimate® XB-C18柱,流动相采用乙腈-水系统,梯度洗脱;柱温30 ℃;检测波长:川续断皂苷VI为212 nm,补骨脂素、异补骨脂素为246 nm。结果 3种成分均能达到基线分离,川续断皂苷VI的进样量在144.1~5 764.0 ng(r=0.999 6)、补骨脂素在5.4~215.2 ng(r=0.998 0)、异补骨脂素在6.6~265.6 ng(r=0.998 5)与峰面积线性关系良好,平均回收率分别为98.11%、97.86%、98.22%,RSD均小于2.0%。结论 建立的方法操作简便、分离良好、数据准确、灵敏度高、工作效率好,可用于仙灵骨葆胶囊的多指标成分定量测定。
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[Abstract]
Objective To determine asperosaponin VI, psoralen, and angelicin in Xianling Gubao Capsules (XGC) via multi- wavelength HPLC method. Methods Separation was carried out on Welch Ultimate® XB-C18 column. The mobile phase was acetonitrile-water system and a linear gradient elution was used. The column temperature was 30 ℃. The detection wavelength for asperosaponin VI was set at 212 nm, those for psoralen and angelicin were set at 246 nm. Results Three components reached baseline separation, the linearity was good when sample volumes were in the ranges of 144.1—5 764.0 for asperosaponin VI (r = 0.999 6), 5.4—215.2 (r = 0.998 0) for psoralen, and 6.6—265.6 ng (r = 0.998 5) for angelicin. The average recoveries of asperosaponin VI, psoralen, and psoralen were 98.11%, 97.86%, and 98.22%, respectively. The RSDs of recoveries were all less than 2.0%. Conclusion The method is simple and accurate and has good separation, with high sensitivity and good efficiency for the determination of more-index components in XGC.
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