目的 筛选灰毡毛忍冬Lonicera macranthoides基因表达分析的内参基因，研究其Mads-box基因家族AGL15基因（LmAGL15）的时空表达特性。方法 克隆灰毡毛忍冬内参基因18 S rRNA、Ubiquilin、Actin和Efl-β的基因片段，评价了4个基因在不同部位叶、茎和花蕾，以及4个基因在灰毡毛忍冬生长不同时期的稳定性，筛选内参基因，分析LmAGL15基因的时空表达特性。结果 18 S rRNA表达最稳定，适宜作为内参基因，叶片、茎的LmAGL15基因相对表达量较低，花蕾相对表达量较高。花蕾不同发育时期，LmAGL15的相对表达量呈现逐渐降低的变化，20 d后LmAGL15的相对表达量最低，然后依次是花蕾初期（15 d）、青绿色花蕾期（10 d）、绿白色花蕾期（5 d）。结论 18 S rRNA是适宜的内参基因。叶片、茎和花蕾中，LmAGL15的相对表达量差异明显。花蕾发育不同时期，LmAGL15的相对表达量呈逐渐降低的变化，与花蕾开放变化规律相似。

Objective To screen the reference genes of Lonicera macranthoides for gene expression analysis and to study the spatio-temporal expression characteristics of LmAGL15 which was a member of Mads-Box family. Methods In this study, 18 S rRNA, Ubiquilin, Actin and Efl-β of L. macranthoides were cloned and the stabilities of the four housekeeping genes were evaluated in different positions (leaves, stems, and buds) and different periods of bud development. In addition, the spatio-temporal expression of LmAGL15 gene was analyzed. Results 18 S rRNA was the most suitable reference gene for spatio-temporal expression analysis in L. macranthoides; The relative expression of LmAGL15 was low in leaves and stems, and that in buds was higher. Conclusion 18 S rRNA is the most suitable reference gene in L. macranthoides. The relative expression of LmAGL15 changes significantly in leaves, stems, and buds.

国家自然科学基金资助项目（81203007）；湖南省自然科学基金资助项目（13JJ9010）；湖南省教育厅科技创新平台（14K071）；湖南省研究生科研创新项目（CX2014B368）：湖南省研究生科研创新项目（CX2014B368）；湖南省“中药学”重点学科建设项目资助（湘教通[2011]76号）；国家中医药管理局“药用植物学”重点学科资助（国中医药发[2009]30号）