目的 观察牡丹皮、赤芍与白芍对急性血瘀模型大鼠的影响，揭示三者在功效方面的异同，并探讨牡丹皮、赤芍与白芍活血功效的机制。方法 大鼠sc盐酸肾上腺素结合冰水浸泡制作急性血瘀模型，以丹参注射液为阳性对照，观察牡丹皮、赤芍与白芍对血液流变学、凝血功能指标、血小板系统、血清中血栓素B₂（TXB₂）、6-酮-前列腺素（6-keto-PGF_1α）的影响。结果 与模型组相比，牡丹皮能够延长急性血瘀模型大鼠的凝血酶原时间（PT）、凝血酶时间（TT）（P<0.05），显著抑制血小板黏附（P<0.01），且上调血小板内皮型一氧化氮合酶（eNOS）与Ser1177位磷酸化的eNOS（p-eNOS）蛋白表达水平（P<0.01、0.001），减少血清中TXB₂（P<0.05）及增加6-keto-PGF_1α的量（P<0.01）；赤芍能够显著改善不同切变率下血瘀模型大鼠的全血黏度，延长部分活化凝血活酶时间（APTT）、PT、TT（P<0.05、0.01），降低血小板黏附率（P<0.05），减少血清中TXB₂的量（P<0.01）、增加6-keto-PGF_1α的量（P<0.01），上调血小板eNOS、p-eNOS蛋白表达水平（P<0.05）；白芍则对血瘀模型大鼠的全血黏度的影响并不显著，但能够显著延长APTT、TT（P<0.05），降低血小板黏附率（P<0.05），增加血清中6-keto-PGF_1α的量（P<0.05），同时显著增加血小板eNOS、p-eNOS蛋白表达水平（P<0.05）。结论 牡丹皮、赤芍和白芍均具有不同程度改善血液流变性、凝血系统、抑制血小板黏附率、增加血小板eNOS、p-eNOS蛋白表达水平，但牡丹皮、赤芍较白芍活血化瘀作用强。

Objective To investigate the effects of Moutan Cortex, Paeoniae Rubra Radix, and Paeoniae Alba Radix on the acute blood stasis model rats, and reveal their mechanism and differences in efficacy. Methods The actue blood stasis model of rats was induced by sc injecting adrenaline hydrochloride and soaking in ice water. Salvia Injection was used as the positive control drug. The changes of viscosity of whole blood and plasma, thrombin activity, blood platelet system, and the contents of TXB₂ and 6-keto-PGF_1α were detected in the acute blood stasis model rats. Results Compared with the model group, Moutan Cortex obviously extended PT, and TT (P<0.05), inhibited platelet adhesion rate (P<0.01), reduced TXB₂ (P<0.01), increased 6-keto-PGF_1α in serum (P<0.01), and regulated eNOS and p-eNOS expression (P<0.01, 0.001). Paeoniae Rubra Radix significantly improved whole blood viscosity at different shear rates, extended APTT, TT, and PT (P<0.05, 0.01), reduced platelet adhesion rate (P<0.05), decreased TXB₂, increased 6-keto-PGF_1α in serum (P<0.01), and increased eNOS and p-eNOS expression (P<0.05). Paeoniae Alba Radix had no significant effect on hemorheology, but could significantly prolong APTT and TT (P<0.05), reduce platelet adhesion rate (P<0.05), increase the content of 6-keto-PGF_1α in serum (P<0.05) and increase the eNOS and p-eNOS expression in platelet. Conclusion All Moutan Cortex, Paeoniae Rubra Radix, and Paeoniae Alba Radix have varying degrees on hemorheology, coagulation system, platelet system, but Moutan Cortex and Paeoniae Rubra Radix have the better role in promoting blood circulation compared with Paeoniae Alba Radix.

国家自然科学基金资助项目（81202906）；江苏省自然科学基金（BK2012491）；中央本级重大增减支项目（2060302）；国家自然基金重点项目（81130070）