目的 建立HPLC法同时检测大黄Rheum officinale叶中没食子酸、番泻苷B、大黄酚-1-O-葡萄糖苷、大黄素-8-O-葡萄糖苷、芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚9 种成分,探索合理开发利用大黄叶的科学依据。方法 采用武本C₁₈色谱柱(250 mm×4.6 mm,5 μm),甲醇-0.2%乙酸水作为流动相,梯度洗脱,体积流量1 mL/min,柱温30 ℃,检测波长260 nm。结果 没食子酸、番泻苷B、大黄酚-1-O-葡萄糖苷、大黄素-8-O-葡萄糖苷、芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚分别在20.2~606.0、79.3~2 379.0、10.1~301.8、14.8~443.7、0.7~2.2、0.13~3.9、12.4~372.0、38.8~1 164.0、6.2~185.4 ng与峰面积良好的线性关系(r>0.999 6);9种成分的平均回收率为95.76%~98.64%,RSD为1.46%~2.43%。结论 该方法简便、准确,分离效果好,所测为大黄叶中化学成分结果真实、可靠,可为其合理开发利用提供参考依据。

Objective To establish an HPLC method for determing nine components from the leaves of Rheum officinale, such as gallic acid, senna glycosides B, rhubarb phenol-1-O-glucoside, emodin-8-O-glucoside, aloe-emodin, rhein, emodin, rhubarb, and emodin methyl ether, as well as to explore the scientific evidence for reasonable exploitation and utilization of medicinal rhubarb leaf. Methods Takeing C₁₈ chromatographic column (250 mm × 4.6 mm, 5 microns), methanol-0.2% acetic acid water as mobile phase, gradient elution and flow rate of 1 mL/min, column temperature 30 ℃, and detection wavelength of 260 nm. Results Gallic acid, senna glycosides B, rhubarb phenol-1-O-glucoside, emodin-8-O-glucoside, aloe-emodin, rhein, emodin, rhubarb, and emodin methyl ether had the good linear relationship, respectively in the range of 20.2-606.0, 79.3-2 379.0, 10.1-301.8, 14.8-443.7, 0.7-2.2, 0.13-3.9, 12.4-372.0, 38.8-1 164.0, 6.2-185.4 ng (r > 0.999 6); The nine kinds of the ingredients of the average recovery were 95.76% - 95.76%, RSD was 1.46% - 2.43%. Conclusion This method is simple and accurate, and with good effect and reliable results for the separation and determination of the components from the leaves of R. officinale, which can provide the reference for its rational development and utilization.

中医药公共卫生专项"国家基本药物所需中药原料资源调查和监测项目"(财社[2011]76号);中医药行业科研专项"我国代表性区域特色中药资源保护利用"(201207002);陕西省教育厅资助项目(13JS030,14JK1204)