目的 剖析浑源黄芪根际微生物菌群结构特征,为研究道地黄芪品质形成提供科学依据。方法 采用核糖体内转录间隔序列法(Automated ribosomal intergenic spacer analysis,ARISA)建立微生物菌群指纹图谱,分别用软件GeneMarker 2.2.0和PAST对获得的原始图谱进行加工处理与分析。结果 与非根际土相比,种植2～3年的浑源黄芪根际微生物菌群结构发生了明显改变,主要表现为相似度降低和多样性增加;种植5年的微生物菌群结构与非根际菌群间相似度无显著差异,多样性趋于一致。与其他产地相比,浑源黄芪核心群体分类操作单元(OTUs)数较低,但相对丰富度较高。结论 浑源黄芪根际微生物菌群结构与种植年限有关,且明显不同于其他产地。

Objective To characterize rhizospheric microbial flora of Hunyuan Astragali Mongolici Radix (HAMR) and to provide scientific evidence for understanding quality formation of the herb. Methods The soil microbial flora were fingerprinted by automated ribosomal intergenic-spacer analysis (ARISA). The raw data were processed and analyzed by GeneMarker and PAST, respectively. Results Compared with non-rhizospheric soil flora, the rhizospheric flora from 2- and 3-year-old herbs shifted obviously, and the similarities were decreased but diversities increased. Meanwhile, the flora from 5-year-old herbs had no significant similarity difference to non-rhizospheric ones and their sample rare fraction curves were more consistent with each other. When compared to the ones from other production areas, the flora from HAMR had relatively lower core OTUs but had significantly higher abundance of core OTUs shared by all areas. Conclusion The structure of rhizospheric microbial flora of HAMR is associated with the herb's age and is quite different from thoes from other areas.

教育部第48批“留学回国人员科研启动基金”;山西省回国留学人员科研资助项目(2013-021);山西省科技厅基础研究面上项目(2014011047-3);第56批中国博士后科学基金面上项目(2014M560170)