[关键词]
[摘要]
目的 研究莛子藨Triosteum pinnatifidum地上部分的化学成分。方法 利用硅胶、Sephadex LH-20、反相ODS等柱色谱技术进行分离和纯化,综合运用质谱、核磁共振波谱方法鉴定化合物结构。结果 从莛子藨地上部分乙醇提取物中分离得到10个化合物,包括2个单萜二糖苷:(E)-(6S)-8-[α-L-arabinopyranosyl-(1→6)-β-D-glucopyranosyloxy]-2,6-dimethyloct-2- eno-1,3"-lactone(1)、urceolide(2)和8个酚类化合物:2,3-二氢穗花杉双黄酮(3)、芹菜素4'-O-β-D-葡萄糖苷(4)、芹菜素7-O-β-D-葡萄糖苷(5)、原儿茶酸(6)、没食子酸乙酯(7)、tachioside(8)、icariside F2(9)、icariside E4(10)。结论 除化合物2外,其他化合物均为首次从本属植物中分离得到。化合物1结构中的阿拉伯糖应更正为1C4式的α-L-阿拉伯糖。
[Key word]
[Abstract]
Objective To study the chemical constituents in the aerial parts of Triosteum pinnatifidum. Methods The constituents were isolated and purified by silica gel, Sephadex LH-20, and ODS column chromatography. Their structures were elucidated by spectroscopic methods including 1D and 2D NMR experiments and HR-ESI-MS analyses. Results Ten compounds were isolated from the aerial parts of T. pinnatifidum, among them there were two monoterpene diglycosides and eight phenols. They were identified as (E)-(6S)-8-[α-L-arabinopyranosyl-(1→6)-β-D-glucopyranosyloxy]-2,6-dimethyloct-2-eno-1,3"-lactone (1), urceolide (2), 2,3-dihydroamentoflavone (3), apigenin-4'-O-β-D-glucoside (4), apigenin-7-O-β-D-glucoside (5), protocatechuic acid (6), ethyl gallate (7), tachioside (8), icariside F2 (9), and icariside E4 (10). Conclusion All the compounds except compound 2 are isolated from the plants of Triosteum Linn. for the first time. The arabinopyranosyl moiety of compound 1 is revised as 1C4 α-L-arabinopyranose.
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[基金项目]
教育部新世纪优秀人才支持计划资助项目(NCET-09-0589)