[关键词]
[摘要]
目的 响应曲面法优化黄花败酱总皂苷(TSPS)的提取工艺及研究TSPS促进大鼠离体肠平滑肌收缩活性。方法 以齐墩果酸为对照品,采用香草醛-冰醋酸显色,利用紫外分光光度计在550 nm处测定TSPS的量。以TSPS提取率作为响应值,在单因素试验的基础上采用响应曲面法考察提取溶剂、料液比、提取时间及其交互作用对TSPS提取效果的影响。并且测定了优化提取工艺过程中获取的不同TSPS提取物的促肠平滑肌收缩活性,结合生物活性全面评价TSPS提取工艺。结果 TSPS的最佳提取工艺条件为提取溶剂75%乙醇,料液比1:12,提取时间为1.5 h,提取温度90 ℃、提取2次。在此工艺条件下TSPS的提取率达到10.96 mg/g,其对大鼠离体肠平滑肌的收缩作用强弱表现为结肠> 回肠> 空肠> 十二指肠,质量浓度达0.32 mg/mL时促进离体肠收缩效果最佳。结论 经优化后的TSPS提取工艺条件具有提取率高,提取工艺高效、稳定等特点,且TSPS具有显著的促进大鼠离体肠平滑肌收缩功能,其功效与提取率呈正相关。
[Key word]
[Abstract]
Objective To optimize the extraction technology for total saponins from Patrinia scabiosaefolia(TSPS) by response surface method. At the same time, the function of promoting intestinal peristalsis by total saponins was investigated. Methods UV spectrophotometric method was used to determine the contents of total saponins in P. scabiosaefolia at the wavelength of 510nm by taking oleanolic acid as standard substance, and vanillin acetic acid as chromogenic reagent. The extraction rate of total saponins was used as index to evaluate the technology based on single factor test, in which three factors were considered the concentration of extraction solvent, solid-liquid ratio, extraction time, and their interaction on extraction were studied by response surface method. The contraction effect and signal changes of total saponins extract of P.scabiosaefolia on intestinal smooth muscle were recordedby tension transducer to the BL-420 biological experimental multi-channel physiological signal acquisition and processing system. Results The best extraction conditions were as follows: ethanol concentration of 75% alcohol, solid-liquid ratio of 1:12, extraction time for 1.5 h, twice and extraction temperature of 90℃. The optimized extraction rate could reach 10.96 mg/g. P.scabiosaefolia extracts obtained using the optimized method showed significantly contraction effect on rat intestinal smooth muscle and the results were displayed as: colon> colon> jejunum> duodenum. The concentration at 0.32 mg/mL has the best effect of gastrointestinal contraction. Conclusion The optimized extraction technology is stable and effective, and with higher extraction rate. The extract of P. scabiosaefolia has a significant promotion effect on intestinal smooth muscle of rat, which shows positive correlation with the rate of extract.
[中图分类号]
[基金项目]