目的 建立败酱草的HPLC指纹图谱。方法 采用Orca C₁₈柱(250 mm×4.6 mm,5 μm);检测波长230 nm;柱温35 ℃。体积流量1 mL/min,以乙腈-0.05%磷酸溶液系统进行梯度洗脱,测定了败酱草药材的HPLC指纹图谱。结果 在选定的色谱条件下,得到败酱草的HPLC指纹图谱,标定出8个共有峰,且方法学考察符合规定。聚类分析表明,I类药材BJ1、BJ2、BJ3、BJ4、BJ5、BJ6、BJ7、BJ8、BJ9、BJ10、BJ13、BJ14各批败酱草药材与对照指纹图谱间的相似度为0.987～0.907,表明各批次药材之间具有较好的一致性;II类药材BJ11、BJ12、BJ15批败酱草药材与对照指纹图谱的差异较大。结论 该方法准确可靠、简便快捷,可作为评价不同产地、不同品种的败酱草药材质量的有效手段。

Objective To establish the HPLC fingerprint of herbs of Patriniae Herba. Methods The fingerprints of Patriniae Herba were built using Orca C₁₈ column and acetonitrile-0.05%phosphoric acid as mobile phase. The flow rate was 1.0 mL/min. The detecting wavelength was set at 230 nm. The temperature of column was at 35℃. Results Under the selected spectrum conditions, HPLC fingerprint of herbs of Patriniae Herba was established, and eight public peaks were shown in the HPLC fingerprint. The methodological study met the required standards. Cluster analysis showed that class I medical BJ1, BJ2, BJ3, BJ4, BJ5, BJ6, BJ7, BJ8, BJ9, both, BJ13, BJ14 each batch Patriniae Herba and control the similarity between fingerprints was 0.987—0.907, indicating that there are good consistency between batches of medicinal materials; class II medical BJ11, BJ12, and BJ15 group of Patriniae Herba and control fingerprint differences larger. Conclusion The method is accurate and reliable, simple and efficient, and can be used as the evaluation of Patriniae Herba from different origins and with different varieties.