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目的 采用高效液相色谱-电喷雾质谱联用技术(HPLC-Q-TOF-MS)对甘草Glycyrrhiza uralensis 50%甲醇提取物中的化学成分进行分析鉴定。方法 Diamonsil II C18(250 mm×4.6 mm,5 μm)反相HPLC梯度洗脱法分离各主要成分;电喷雾电离源经正、负离子模式对色谱流出物进行检测,四级杆飞行时间串联质谱法对各主要色谱峰进行归属;选择甲酸钠溶液为内标矫正。结果 通过二级高分辨质谱分析结合对照品数据及相关文献,从甘草50%甲醇提取物中共鉴定出40个化学成分,包括28个黄酮类、11个三萜皂苷类和1个香豆素类化合物。结论 利用液质联用技术鉴定甘草中的化合物并提供结构信息,为快速分析甘草药材的物质基础提供可行方法。
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[Abstract]
Objective To analyze the main chemical constituents of Glycyrrhizae Radix et Rhizoma by HPLC-Q-TOF-MS in the positive and negative ions mode. Methods The chromatographic fingerprint was obtained with Diamonsil II C18 column (250 mm × 4.6 mm, 5 μm) and gradient elution with 0.05% H2O-formic acid (A)-acetonitrile (B), and the flow rate was 1 mL/min. The column temperature was maintained at 35 ℃. The detection wavelength was of 200?600 nm. Positive and negative ions MS information of Bruker Daltonics 1200 HPLC-Q-TOF was coupled with elemental analysis and compared with the literature data to analyze the compounds information. Results Combined with the accurate relative molecular mass of compounds provided by HPLC-Q-TOF-MS, 40 compounds were identificated in the ethanolic extract from Glycyrrhizae Radix et Rhizoma, which include 28 flavonoids, 11 triterpenoids saponins, and one coumarin. Conclusion The method provides the technical support for the quality control of Glycyrrhizae Radix et Rhizoma, and contributes the reference data to elucidating the potential basis of Glycyrrhizae Radix et Rhizoma.
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