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[摘要]
目的 利用HPLC-Q-TOF-MS方法研究柴胡中的化学成分。方法 采用Agilent 1200 HPLC Diamonsil II C18色谱柱(250 mm×4.6 mm,5 μm),以0.05%甲酸水溶液(A)-乙腈(B)为流动相,梯度洗脱,体积流量为1.0 mL/min,柱温35 ℃,以200~600 nm全扫描方式进行检测,利用正、负离子质谱信息和元素分析,结合对照品对比质谱数据,并与相关文献数据对照,分析柴胡的主要化学成分。结果 从柴胡中检测出24个峰,分析其质谱裂解规律,并参考文献结合对照品对照,共鉴定了21个化合物,包括3个酚酸类化合物,4个黄酮类化合物,14个三萜皂苷类化合物。其中4个化合物:异绿原酸A(5)、异绿原酸B(6)、7,3'-二甲氧基槲皮素(8)、5-羟基-7-乙酰氧基黄酮(24)为首次从柴胡中报道。结论 HPLC-Q-TOF-MS方法可用于快速分析柴胡中化学成分组成,为建立快速、准确的质量评价方法以及柴胡药材的应用开发提供了参考。
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[Abstract]
Objective To investigate the chemical constituents in extract of Bupleuri Radix using HPLC-Q-TOF-MS. Methods The HPLC separation achieved on Agilent 1200 HPLC Diamonsil II C18 column (250 mm × 4.6 mm, 5 μm) was used with a mobile phase of 0.05% H2O-formic acid (A) and acetonitrile (B), the flow rate was 1.0 mL/min, the column temperature was 35 ℃, and the detection wavelength was 200?600 nm. Positive and negative ions MS information and element analysis, via comparing MS data with those of the standard compounds and coupled with the related literature were used to analyze the main chemical constituents of BupleuriRadix. Results Twenty-one chemical constituents were identified, including three phenolic acid, four flavonoids, and fourteen triterpenoid saponins, and isochlorogenic acid A (5), isochlorogenic acid B (6), 7,3'-di-O-methylquercetin (8), and 5-hydroxy-7-acetoxyflavone (24) were separated from Bupleuri Radix for the first time. Conclusion It is used to quickly analyze the chemical constituents from Bupleuri Radix by establishing a rapid accurate evaluation method using HPLC-Q-TOF-MS, which could provide the references for the application development.
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