[关键词]
[摘要]
目的 建立秦艽中马钱苷酸、獐牙菜苦苷、6'-O-β-D-葡萄糖基龙胆苦苷、龙胆苦苷、獐牙菜苷、异荭草苷和异牡荆苷测定的UPLC方法。方法 采用色谱柱ACQUITY UPLC® BEH C18(50 mm×2.1 mm,1.7μm);流动相甲醇-0.04%磷酸水溶液梯度洗脱,体积流量0.3 mL/min;检测波长242 nm;柱温30℃。结果 马钱苷酸、獐牙菜苦苷、6'-O-β-D-葡萄糖基龙胆苦苷、龙胆苦苷、獐牙菜苷、异荭草苷和异牡荆苷分别在2.100~537.100、1.050~270.000、0.920~236.000、11.100~2830.000、0.750~192.000、0.167~102.000、0.216~52.800μg进样量与峰面积积分值线性关系良好(r ≥ 0.9995),平均回收率97.83%~100.08%,RSD ≤ 3.76%。结论 UPLC较HPLC更简便、更有效,可用于秦艽中7种指标成分的同时测定。
[Key word]
[Abstract]
Objective An ultra performance liquid chromatographic (UPLC) method was developed for simultaneously determining seven constituents, such as loganic acid, swertiamarin, 6'-O-β-D-glucosyl gentiopicroside, gentiopicroside, sweroside, isoorientin, and isovitexin, from the medicinal plants of Gentiana macrophylla. Methods The separation was performed on an Acquity UPLC® BEH C18 column (50 mm×2.1 mm, 1.7 μm) through a gradient elution of methanol -0.04% aqueous phosphorite at a flow rate of 0.3 mL/min. The detection wavelength was 242 nm, and the column temperature was set at 30℃. Results For the seven analytes, loganic acid, swertiamarin, 6'-O-β-D-glucosyl gentiopicroside, gentiopicroside, sweroside, isoorientin, and sovitexin, a good linearity (r≥0.9995) was obtained in the range of 2.100-537.100, 1.050-270.000, 0.920-236.000, 11.100-2830.000, 0.750-192.000, 0.167-102.000, and 0.216-52.800 μg, respectively. Their average recoveries (n=6) were 97.83%-100.08%, respevtively, with RSD values less than or equal to 3.76%. Conclusion The UPLC method is simpler and more effective than HPLC, and can be used for the simultaneous determination of seven indicative constituents in medicinal plants of G. macrophylla.
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[基金项目]
中央本级重大增减支项目"名贵中药资源可持续利用能力建设"子课题"秦艽全国生产区划研究"(20603020212);"秦艽质量特征和商品规格研究"(20603020110);2015年甘肃省高等学校科学研究项目"濒危中(藏)药麻花秦艽遗传分子与药效成分相关性研究"(2015A-098)