目的 研究平卧菊三七Gynura procumbens全草的化学成分。方法 利用反复硅胶柱色谱、Sephadex LH-20凝胶柱色谱、中压柱色谱及半制备高效液相色谱等方法分离纯化,通过NMR、MS等鉴定化合物结构。结果 从平卧菊三七全草的70%乙醇提取物中分离得到16个化合物,分别鉴定为槲皮素(1)、芹菜素(2)、木犀草素(3)、山柰酚(4)、黄芪苷(5)、山柰酚-5-O-(6"-O-乙酰基)-β-D-吡喃葡萄糖苷(6)、7-甲醚黄芩素(7)、4-甲氧基肉桂酸(8)、苄基葡萄糖苷(9)、2-苯乙基-O-β-D-吡喃葡萄糖苷(10)、3,5-二咖啡酰基奎宁酸甲酯(11)、3,5-二咖啡酰基奎宁酸乙酯(12)、3,4-二咖啡酰基奎宁酸甲酯(13)、4,5-二咖啡酰基奎宁酸甲酯(14)、原儿茶酸(15)和丁香酚苷(16)。结论 化合物7、8、12、14和16为首次从菊三七属植物中分离得到,化合物3、6、9~11和13为首次从该植物中分离得到。

Objective To investigate the chemical constituents from the whole plants of Gynura procumbens. Methods The chemical constituents were isolated and purified by repeated silica gel column chromatography, Sephadex LH-20 gel column chromatography, medium pressure column chromatography, and semi-preparative HPLC, and their structures were elucidated by chemical properties and spectroscopic analyses. Results Sixteen compounds were identified to be quercetin (1), apigenin (2), luteolin (3), kaempferol (4), astragaline (5), kaempferol-5-O-(6"-O-acetyl)-β-D-glucopyranoside (6), negletein (7), 4-methoxycinnamic acid (8), benzyl-O-β-D-glucopyranoside (9), 2-phenylethyl-O-β-D-glucopyranoside (10), 3,5-dicaffeoylquinic acid methyl ester (11), 3,5-dicaffeoylquinic acid ethyl ester (12), 3,4-dicaffeoylquinic acid methyl ester (13), 4,5-dicaffeoylquinic acid methyl ester (14), protocatechuic acid (15), and eugenol glucoside (16). Conclusion Compounds 7, 8, 12, 14, and 16 are obtained from the plants in Gynura Cass. for the first time, and compounds 3, 6, 9-11 and 13 are obtained from this plant for the first time.

江西省青年科学家培养对象计划项目(20142BCB23022);江西中医药大学重点学科建设基金