[关键词]
[摘要]
目的 研究苹果Malus pumila树枝的化学成分。方法 采用硅胶柱色谱、Sephadex LH-20柱色谱、ODS柱色谱以及HPLC等方法进行分离纯化,根据其理化性质和波谱数据鉴定化合物结构。结果 从苹果树枝乙醇提取物的醋酸乙酯部位分离纯化得到11个化合物,分别鉴定为根皮素(1)、erlodictyol(2)、8-甲氧基山柰酚-7-O-鼠李糖苷(3)、槲皮素(4)、6-甲氧基槲皮素(5)、对原儿茶酸甲酯(6)、槲皮素-3-O-呋喃阿拉伯糖苷(7)、根皮苷(8)、金鱼草素-6-O-β-D-葡萄糖苷(9)、槲皮素-3-O-D-木糖(10)、3,4-二羟基苯甲酸乙酯(11)。结论 化合物2和9为首次从蔷薇科植物中分离得到,化合物11为首次从该属植物中分离得到,化合物3为首次从该植物中分离得到。
[Key word]
[Abstract]
Objective To study the chemical constituents from the branches of Malus pumila. Methods Compounds were isolated and purified on many kinds of column chromatography, such as silica gel, Sephadex LH-20, ODS, and PHPLC. The structures of the obtained compounds were identified by their physical properties and spectroscopic data. Results Eleven compounds were separated and purified from the ethyl acetate extract from the branches of M. pumila. They were identified to be phloretin (1), erlodictyol (2), 8-methoxyl-kaempferol-7-O-rhamnoside (3), quercetin (4), 6-methoxy-quercetin (5), protocatechuic acid methyl ester (6), quercetin-3-O-arabinofuranosidase (7), phlorizin (8), aureusidin-6-O-β-D-glucoside (9), quercetin-3-O-D-xylose (10), and 3,4-dihydroxybenzoic acid ethyl ester (11). Conclusion Compounds 2 and 9 are firstly reported from the plants of Rosaceae. Compound 11 is isolated from this genus and compound 3 is obtained from this plant for the first time.
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[基金项目]
国家自然科学基金资助项目(31201345);天津市自然科学基金资助项目(13JCYBJC24400);中国博士后科学基金项目(2014M562674)