目的 研究配伍比例及pH值环境对黄连-大黄配伍后4种生物碱成分溶出的变化规律。方法 在一测多评法的基础上,采用HPLC法测定小檗碱、巴马汀、黄连碱及表小檗碱的量。结果 黄连-大黄合煎组及分煎合并组中4种生物碱的量及相对溶出率随着大黄比例增加均有相似的降低趋势,2组黄连与大黄配伍比例为1:2时,4种生物碱的量及相对溶出率均达到最大值;且配伍比例与4种生物碱的量及相对溶出率无效应关系;盐酸溶液单煎黄连组pH值为5.2时,4种生物碱的量及相对溶出率达到最大值,pH值与4种生物碱的量及相对溶出率无效应关系。结论 pH值环境对黄连-大黄配伍后4种生物碱成分的量有影响,且不同比例黄连-大黄配伍后形成的pH值环境与用盐酸调配的pH值环境对生物碱成分的溶出产生的影响不一致。

Objective To study the dissolution changes of four alkaloid contents in the comptability of Coptidis Rhizoma and Rhei Radix et Rhizoma based on compatability ratio and pH value environment. Methods To determine the contents of berberine, palmatine, coptisine, and epiberberine by HPLC based on the quantitative analysis of multi-components by single marker (QAMS) method. Results Contents of four kinds of alkaloids and their relative dissolution rates in the co-decoction or mixture of single decoction of Coptidis Rhizoma and Rhei Radix et Rhizoma have a similar reduction trend with the increasing of the ratio of Rhei Radix et Rhizoma. When the proportion of Coptidis Rhizoma and Rhei Radix et Rhizoma was 1:2, the contents and their relative dissolution rates reached the maximum value, while the dispensing ratio has no effect relationship with the contents and the relative dissolution rates. When the pH value environment of the single decoction of Coptidis Rhizoma in the hydrochloric acid solution was 5.20, the pH value environment had no effect relationship with the contents of four alkaloids and relative dissolution rate. Conclusion There were influences on the contents of the four alkaloids and their relative dissolution rates in the compatability of Coptidis Rhizoma and Rhei Radix et Rhizoma. And both dispensing ratios and pH value environment in the different ratios of Coptidis Rhizoma and Rhei Radix et Rhizoma have the inconsistent influence on the alkaloids with the pH value environment by hydrochloric acid.

中国中医科学院自主选题(Z02063);2009年中医药行业科研专项(200907001-5)