目的 采用高效液相色谱-二极管阵列检测方法,建立昆仑雪菊中绿原酸、芦丁、槲皮素和木犀草素的测定方法。方法 色谱柱为安捷伦HC-C₁₈柱(250 mm×4.6 mm,5μm);流动相为甲醇-0.01%磷酸水溶液,梯度洗脱;检测波长315 nm;柱温30℃;体积流量1.0 mL/min。结果 昆仑雪菊中绿原酸、芦丁、槲皮素和木犀草素分别在2.0~60μg/mL(r=0.9998)、5.0~100μg/mL(r=0.9999)、1.0~40μg/mL(r=0.9999)和0.5~40μg/mL(r=0.9999)呈良好的线性关系,平均回收率分别为102.4%(RSD为1.05%)、98.6%(RSD为1.28%)、103.6%(RSD为0.95%)、101.2%(RSD为1.12%)。结论 该方法操作简便、易行、稳定,具有良好的灵敏性、重现性和回收率,可用于昆仑雪菊药材的质量控制。

Objective The high performance liquid chromatography diode array detection method was used to establish the determination methods for the contents of chlorogenic acid, rutin, quercetin, and luteolin in Kunlun snow daisy (Coreopsis tinctoria, Coreopsis Tinctoriae Folium). Methods Agilent HC-C₁₈ column (250 mm×4.6 mm, 5 μm) was used. The mobile phase was CH₃OH (A)-0.01% H₃PO₄ (B) for the gradient elution with flow rate of 1.0 mL/min, the detection wavelength was 315 nm and the column temperature was 30℃. Results There was a linear correlation between the concentration of chlorogenic acid 2.0-60 μg/mL (r=0.999 8), rutin 5.0-100 μg/mL (r=0.999 9), quercetin 1.0-40 μg/mL (r=0.999 9), and luteolin 0.5-40 μg/mL (r=0.999 9). The average recovery rates of chlorogenic acid, rutin, quercetin, and luteolin were 102.4% (RSD=1.05%, n=5), 98.6% (RSD=1.28%, n=5), 103.6% (RSD=0.95%, n=5), and 101.2% (RSD=1.12%, n=5), respectively. Conclusion The analysis method is simple, rapid, reproducible, accurate, and reliable. It could be used to identify and evaluate the quantitative determination of chlorogenic acid, rutin, quercetin, and luteolin contents in Kunlun snow daisy.

自治区高校科研计划重点项目(XJEDU2011147)