目的 研究光叶丁公藤Erycibe schmidtii藤茎的化学成分。方法 利用正、反相硅胶色谱柱、Sephadex LH-20色谱及半制备高效液相色谱等方法分离纯化,通过核磁共振波谱分析方法鉴定结构。利用NO相对生成率法对量较多的化合物进行抗炎活性检测。结果 从光叶丁公藤藤茎95%乙醇提取物中分离得到12个化合物,分别鉴定为咖啡酸(1)、N-反式-对羟基苯乙基阿魏酰胺(2)、绿原酸(3)、绿原酸甲酯(4)、4-O-咖啡酰基奎宁酸(5)、4-O-咖啡酰基奎宁酸甲酯(6)、4,5-O-双咖啡酰基奎宁酸(7)、4,5-O-双咖啡酰基奎宁酸甲酯(8)、3,5-O-双咖啡酰基奎宁酸(9)、3,5-O-双咖啡酰基奎宁酸甲酯(10)、3,4-O-双咖啡酰基奎宁酸(11)、3,4-O-双咖啡酰基奎宁酸甲酯(12)。化合物1 和2 对RAW 264.7细胞释放NO显示出一定的抑制活性。结论 化合物5~7 、9 、11 、12 为首次从该属植物中分离得到,化合物1 、2 、4~12 为首次从该植物中分离得到。化合物1 和2 有一定抗炎活性。

Objective To investigate the chemical constituents of Erycibe schmidtii. Methods The compounds were separated and purified by silica gel and Sephadex LH-20 column chromatography, and semipreparative HPLC. Their structures were determined by nuclear magnetic resonance (NMR) spectroscopy analysis method. The anti-inflammatory activity of some compounds was tested by relative NO productivity. Results Twelve compounds were isolated and identified as caffeic acid (1), N-trans-p-hydroxyphenethyl ferolamine (2), chlorogenic acid (3), chlorogenic acid methyl ester (4), 4-O-caffeoylquinic acid (5), 4-O-caffeoylquinic acid methyl ester (6), 4,5-di-O-caffeoylquinic acid (7), 4,5-di-O-caffeoylquinic acid methyl ester (8), 3,5-di-O-caffeoylquinic acid (9), 3,5-di-O-caffeoylquinic acid methyl ester (10), 3,4-di-O-caffeoylquinic acid (11), and 3,4-di-O-caffeoylquinic acid methyl ester (12). Compounds 1 and 2 showed some inhibitory activity for RAW cells 264.7 releasing NO. Conclusion Compounds 5-7, 9, 11, and 12 are obtained from the plants of Erycibe Roxb. for the first time, while compounds 1, 2, and 4-12 are separated from this plant for the first time. Compounds 1 and 2 exhibit certain anti-inflammatory activity.

四川省杰出青年学术技术带头人培育计划(2014JQ0049)