目的 采用¹H-NMR代谢组学技术结合多元统计分析方法,探讨乙酰苯肼诱导的溶血性贫血大鼠血清中内源性代谢产物的变化以及驴胶补血颗粒的干预作用。方法 采用乙酰苯肼制备大鼠溶血性贫血模型,造模1周后,给药组ig驴胶补血颗粒水溶液(8 g/kg),对照组和模型组分别ig等量蒸馏水,每天1次,持续2周。采集大鼠血清进行¹H-NMR检测,利用代谢组学技术分析血清中内源性代谢产物的变化。结果 与对照组比较,模型组血清中脂质、乳酸以及丙酮水平升高,丙氨酸、缬氨酸、肌酐、磷酸胆碱、甘油磷酸胆碱、氧化三甲胺、甘氨酸以及精氨酸水平下降。给予驴胶补血颗粒后大鼠血清中上述11种内源性差异代谢产物均出现了不同程度的回调,接近对照组水平。结论 驴胶补血颗粒的作用机制主要涉及能量代谢、脂质代谢、肠菌代谢等代谢途径的调节。

Objective To explore the change of endogenous metabolites in serum of rats with erythronoclastic anemia and the intervention of Lvjiao Buxue Granules by using ¹H-NMR metabonomics coupled with multivariate statistical analysis. Methods Acetylphenylhydrazine was used to establish the rat model of erythronoclastic anemia and after a week the rats except control and model groups were given to Lvjiao Buxue Granules(8 g/kg) separately once daily for two weeks. While the rats in the control and model groups were ig given equivalent distilled water respectively. The endogenous metabolites in serum from all of rats were analyzed by ¹H-NMR coupled with multivariate statistical analysis. Results Compared with the control group, the levels of lipids, lactic acid, and acetone in serum of rats in the model group increased while the levels of alanine, valine, creatinine, phosphocholine, glycerophosphocholine, oxidation of three methyl ammonium, glycine, and arginine were decreased. The levels of these endogenous metabolites with significant difference were reversed to normal by ig asminstration of Lvjiao Buxue Granules. Conclusion The mechanism of Lvjiao Buxue Granules on tonifying blood is involved in the pathway of energy metabolomic, lipid metabolism, and intestinal bacteria metabolism.

国家自然科学基金资助项目(81441096);山西省科技创新重点团队(2013131015)