目的 采用HPLC法分析地黄生品及炮制品中8种糖类成分,探索炮制时间对地黄中糖类成分的影响。方法 采用Prevail Carbohydrate ES色谱柱(250 mm×4.6 mm,5μm),体积流量0.8 mL/min,蒸发光散射检测器检测,流动相为乙腈-水,梯度洗脱。结果 果糖、葡萄糖、蔗糖、密二糖、棉子糖、甘露三糖、水苏糖、毛蕊花糖分离良好。在相应质量浓度范围内8种化合物的线性关系良好,r在0.9984~0.9997;平均加样回收率为97.5%~103.2%。不同炮制时间点定量分析结果显示地黄中糖类成分蔗糖、棉子糖、水苏糖和毛蕊花糖在炮制过程中呈下降趋势,而果糖、葡萄糖、密二糖和甘露三糖明显上升。结论 地黄生品与炮制品中糖类比例明显不同,不同炮制时间地黄中糖类成分有不同的变化趋势。

Objective To analyze the contents of eight saccharides in unprocessed and processed Rehmannia glutinosa by HPLC and to investigate the impacts of processing time on saccharides in R. glutinosa. Methods HPLC conditions were as follows:Prevail Carbohydrate ES column(250 mm×4.6 mm, 5μm), flow rate of 0.8 mL/min, ELSD detector, acetonitrile as mobile A and water as mobile B for gradient elution. Results Fructose, glucose, surcrose, melibiose, raffinose, manninotriose, stachyose, and verbascose were separated with good linear relationships within their corresponding concentration ranges, the r values were within 0.9984-0.9997; the average recovery was 97.5%-103.2%. The content analysis at different processing time points showed that surcrose, raffinose, stachyose, and verbascose exhibited decreasing trend during processing, while fructose, glucose, melibiose, and manninotriose exhibited significantly increasing trend. Conclusion The proportions of saccharides in unprocessed and processed R. glutinosa are significantly different, and the results of this study could provide the data support for revealing the processing mechanism of R. glutinosa.