[关键词]
[摘要]
目的 研究离体蒜苗挥发物质对模拟连作胁迫下当归Angelica sinensis 种子发芽特性的影响。方法 采用当归根浸提液处理当归种子模拟连作障碍,用离体蒜苗模拟大蒜挥发物质的化感作用环境,进行当归种子发芽特性研究。结果 当归根浸提液对当归种子的发芽具有明显的抑制作用,且当归根浸提液质量浓度越大,其抑制作用越强;对于未用当归根浸提液处理的当归种子,离体蒜苗挥发物质对当归种子发芽特性的影响基本表现为低质量(蒜苗质量50 g)促进而高质量(蒜苗质量100~200 g)抑制的趋势;对于用当归根浸提液处理过的当归种子,离体蒜苗挥发物质对当归种子发芽特性的影响表现为一定的化感促进作用,当供体蒜苗质量为50~100 g 时,其对当归发芽的促进效应达到极显著水平(P < 0.01),当供体蒜苗质量为200 g 时,促进作用不显著(P < 0.05),但不存在抑制作用。结论 离体蒜苗产生的挥发物质对由当归根浸提液产生的发芽抑制作用具有一定的缓解效应。
[Key word]
[Abstract]
Objective To study the effect of volatile from isolated garlic sprouts on the germination characteristics of Angelica sinensis seeds under simulated continuous cropping stress. Methods Treating the obstacle of A. sinensis seeds under simulate continuous cropping stress with the extract from Angelica Sinensis Radix and using isolated garlic sprouts to simulate garlic volatile allelopathy environment, so as to study the germination characteristics of A. sinensis seeds. Results The extracts from Angelica Sinensis Radix inhibited the germination of A. sinensis seeds, and the higher the concentration was, the stronger the inhibition effects will be. The volatile from the isolated garlic sprouts promoted the germination of A. sinensis seeds, which was not treated with the extracts from Angelica Sinensis Radix at low concentration (50 g isolated garlic sprouts) and inhibited the germination at high concentration (100-200 g isolated garlic sprouts). The isolated garlic sprouts volatile had certain promoting allelopathy on the germination properties of A. sinensis seeds which were treated with the extracts from Angelica Sinensis Radix. When the fresh weight of the donor garlic sprouts was 50-100 g, the promoting effects on A. sinensis seeds germination reached extremely significant level (P < 0.01). When the fresh weight of garlic sprouts was 200 g, the promoting effects were not significant (P < 0.05). Conclusion The volatile from the isolated garlic sprouts could alleviate the germination inhibition by extracts from Angelica Sinensis Radix.
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[基金项目]
国家自然科学基金资助项目(31201176);甘肃省中药现代制药工程研究院科研项目资助