目的 利用ITS 条形码标记技术对当归属药用植物进行鉴别。方法 选取该属32 个物种158 条核糖体内转录间隔区(internal transcribed spacer,ITS)序列进行分子鉴别分析。所得序列运用MEGA 6.0 软件计算种内和种间遗传距离,同时分别构建邻接树(neighbor-joining tree,NJ)、最大似然树(maximum-likelihood tree,ML)和最大简约树(maximum-parsimonytree,MP),分析ITS 序列的鉴别能力和当归属药用植物种间的系统发育关系。结果 当归属物种间ITS 序列最小遗传距离大于种内最大遗传距离,而且系统发育结果显示,每个物种的ITS 基因型序列能较好地聚类为对应于该物种本身的分支,且具有中到高度的支持率,说明ITS 序列能够将当归属物种区分开。结论 ITS 序列可以作为当归属药用植物的DNA 分子条形码标记,将在该属物种的分子鉴定方面发挥重要潜力。

Objective DNA molecular identification of medicinal plants in Angelica L. was carried out based on the internal transcribed spacer (ITS) of ribosomesequence variation. Methods One hundred and fifty-eight individuals of 32 species from Angelica L. were selected, and ITS sequences were used to evaluate the differences among these species. Obtained sequences were analyzed using MEGA6.0 program with the kimura-2-parameter (K2P) model. Meanwhile, phylogenetic trees were constructed, included the neighbor-joining (NJ) tree, the maximum-likelihood (ML) tree, and the maximum-parsimony (MP) tree, and we determined the identification ability of ITS sequences and the phylogenetic relationships among the species in Angelica L. Results The minimum interspecific distance is far greater than the maximum intraspecific distance in ITS region. The phylogenetic analysis showed that the species in Angelica L. can be easily differentiated according to their strong bootstrap support and relationships. Conclusion ITS region as DNA barcode can distinguish the medicinal plants in Angelica L. and would be a foundation for the aid of molecular identification of the species in Angelica L.

国家自然科学基金资助项目(31470400)