[关键词]
[摘要]
目的 建立过敏性鼻炎颗粒中挥发油的毛细管气相色谱指纹图谱,为过敏性鼻炎颗粒的质量控制提供新方法。方法 建立毛细管气相色谱法,采用氢火焰检测器及质谱进行检测分析。HP-5 Phenyl Methyl Siloxane 毛细管色谱柱(30 m×0.32mm,0.25 μm);检测器温度250℃;进样口温度200℃;柱温采取程序升温,初始温度50℃,以3℃/min 升温至140℃,保持2 min,再以10℃/min 升温至200℃,保持2 min。结果 建立了过敏性鼻炎颗粒中挥发油的毛细管气相指纹图谱方法,确定了过敏性鼻炎颗粒中12 个特征峰,并采用GC-MS 对主要色谱峰进行了初步定性。10 批成品指纹图谱相似度在0.90以上。结论 建立的毛细管气相色谱指纹图谱有较好的精密度、重复性和稳定性,适用于过敏性鼻炎颗粒的质量控制。
[Key word]
[Abstract]
Objective To establish the capillary GC fingerprint of Guominxing Biyan Granules and give a new method for quality control. Methods The capillary GC was used. The ingredients of common peaks were analyzed by FID and MS. The HP-5 Phenyl Methyl Siloxane(30 m×0.32 mm, 0.25 μm)was used for capillary column. The chromatography conditions were as follows: the temperature of inlet was 200℃ in splitless sampling, the detector temperature was 250℃, the column initial temperature was 50℃, 3℃/min rose to 140℃, keeping 2 min and 10℃/min rose to 200℃, keeping 2 min. Results Capillary GC fingerprint of volatile oil in Allergic Rhinitis Particles was established. Twelve peaks were selected as the fingerprint peaks of Allergic Rhinitis Particles. The ingredients of common peaks were designated by GC-MS. The similarity between the fingerprint of 10 batches of Allergic Rhinitis Particles Samples were 0.90. Conclusion The established capillary GC fingerprint has desirable precision, reproducibility, and stability, and can be applied to the quality control of Guominxing Biyan Granules.
[中图分类号]
[基金项目]
国家科技部重大新药创制:现代中药创新集群与数字制药技术平台(2013ZX09402203)