[关键词]
[摘要]
目的 分析国内不同产地蒺藜果实之间以及果实与全草之间化学成分的差异。方法 色谱分离采用Acquity HSS T3C18 色谱柱(Waters,100 mm×2.1 mm,1.8 μm),0.1%甲酸水溶液-乙腈为流动相进行梯度洗脱,质谱分析利用四级杆串联飞行时间质谱(Q-TOF MSE);所得数据采用主成分分析法(PCA)及正交偏最小二乘判别分析(OPLS-DA)进行处理,差异标志物通过精确相对分子质量和碎片离子鉴定结构。结果 PCA 结果显示本实验所研究的各产地蒺藜果实之间无显著差异,而蒺藜果实与全草之间存在一定差异性,并利用液质联用技术鉴定了两者之间差异性较大的12 个化学成分,其中9 个主要存在于果实中,另外3 个主要存在于全草中。结论 通过研究国内几个重要产区蒺藜果实之间及果实与全草之间化学成分的差异,为蒺藜资源的合理利用以及临床用药提供理论依据。
[Key word]
[Abstract]
Objective To analyze the chemical differences in the fruits of Tritulus terrestris from different areas in the domestic and explore the potential chemical markers between the fruits and whole plants of T. terrestris. Methods Chromatographic separation was carried out on the Acquity HSS T3 C18 column (100 mm×2.1 mm, 1.8 μm, Waters) with the mobile phase composed of 0.1% formic acid in water-acetonitrile in the gradient elution. A hybrid quadrupole time-of-flight tandem mass spectrometry (Q-TOF MSE) was used for mass spectrometric analysis. The obtained datasets were processed by principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA). Finally, the structures of chemical markers between fruits and whole plants were identified based on their exact mass data and fragmentation characteristics. Results There was almost no difference in fruits of T. terrestris from different areas of China. However, the compounds existed in the fruits were different from those in the whole plants of T. terrestris. Eventually 12 chemical markers between fruits and whole plants of T. terrestris were identified online including nine chemical markers mainly distributed in the fruits and three chemical markers mainly distributed in the whole plants. Conclusion This study could provide the guidance for the utilization of resource and treatments of T. terrestris in clinic by comparative analysis on chemical constituents in the different parts of T. terrestris from different areas.
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[基金项目]
国家自然科学基金面上项目(81373938);北京市自然科学基金面上项目(7152114)