目的 研究鼠曲草Gnaphalium affine的化学成分及其抗氧化活性。方法 采用硅胶柱色谱、Sephadex LH-20柱色谱等方法分离纯化,根据理化性质和波谱数据进行结构鉴定。测定了化合物1、3~11、16对DPPH自由基的清除活性。结果 从鼠曲草95%乙醇部位分离得到了17个化合物,分别鉴定为芹菜素(1)、二氢芹菜素(2)、木犀草素(3)、白杨素(4)、汉黄芩素(5)、豆甾醇(6)、β-谷甾醇(7)、熊果酸(8)、齐墩果酸(9)、19α-羟基熊果酸(10)、2α,3α,19α-trihydroxy-28-norurs-12-ene(11)、α-香树酯醇乙酸酯(12)、β-香树酯醇乙酸酯(13)、patriscabratine(14)、aurantiamide acetate(15)、4'-hydroxydehydrokawain(16)、异香草醛(17)。化合物1和3~5有显著的抗氧化活性。结论 化合物2、4、5、11~15、17为首次从该植物中分离得到。

Objective To study the chemical constituents from Gnaphalium affine and their anti-oxidative activities. Methods Chemical constituents were isolated by column chromatography and semi-prepared HPLC, the structures were elucidated by spectral data and physicochemical properties. DPPH free radical scavenging activities of compounds 1, 3-11, and 16 were determined. Results Seventeen compounds were isolated and respectively identified as apigenin (1), dihydroapigenin (2), luteolin (3), chrysin (4), wogonin (5), stimasterol (6), β-sitosterol (7), ursolic acid (8), oleanolic acid (9), 19α-hydroxyl-oleanolic acid (10), 2α,3α,19α-trihydroxy-28-norurs-12ene (11), α-amyrin acetate (12), β-amyrin acetate (13), patriscabratine (14), aurantiamide acetate (15), 4'-hydroxydehydrokawain (16), and isovanillin (17). Compounds 1 and 3-5 had significant anti-oxidative activities. Conclusion Compounds 2, 4, 5, 11-15, and 17 are isolated from G. affine for the frist time.

黑龙江省自然科学基金资助项目(B201314);黑龙江省教育厅资助项目(12521595);教育部留学回国人员科研启动基金项目(2009361341)