目的 采用RAPD和ISSR分子标记进行野生红景天种间亲缘关系及种内的遗传多样性分析。方法 用CTAB法提取基因组DNA,然后通过筛选得到的11个RAPD及11个ISSR引物对不同采集地的4种野生红景天进行遗传多样性分析。结果 11条RAPD引物共扩增出96条条带,多态性百分比为90.62%;11条ISSR引物共扩增出102条条带,多态性百分比为100%。ISSR多态性的检测能力优于RAPD;聚类分析结果表明,ISSR法、RAPD和ISSR联用法均将17个样品聚为3大类;RAPD将样品聚为4大类。结论 2种标记均可用于红景天属植物种间亲缘关系与种内遗传多样性研究;4种红景天种内存在一定的遗传差异,种间基因流较小。

Objective The interspecific genetic relationships of the plants in Rhodiola L. were analyzed by RAPD and ISSR molecular markers. Methods The genomic DNA was extracted by CTAB method. Eleven RAPD primers and 11 ISSR primers were selected to analyze the genetic diversities of four kinds of wild plants in Rhodiola L., which were obtained from different regions of China. Results A total of 96 bands were amplified by 11 RAPD primers, and the percentage of polymorphism was 90.62%; And 102 bands were amplified by 11 ISSR primers, and the percentage of polymorphism was 100%. So the polymorphism detection ability of ISSR marker is higher than that of RAPD marker. Clustering analysis indicated that the samples were clustered into three categories by ISSR, RAPD + ISSR, and four categories by RAPD. Conclusion Both ISSR and RAPD markers are efficient methods at revealing in interspecific or intraspecific genetic differences and diversity of the plants in Rhodiola L.

国家自然科学基金资助(31270383);山西省自然科学基金(2014011029-1);高等学校博士学科点专项科研基金(20121401120001);林业公益性行业科研专项(201304326)