目的 研究大花红景天Rhodiola crenulata干燥根及根茎的化学成分。方法 利用反复硅胶柱色谱、中压柱色谱等方法分离纯化;采用ESI-MS、¹H-NMR、¹³C-NMR等现代波谱技术进行结构鉴定。结果 从大花红景天醋酸乙酯部位分离得到14个化合物,分别鉴定为3,5-二羟基-3',4',7-三甲氧基黄酮(1)、3,5,7,3'-四羟基黄酮(2)、5,4'-二羟基-7,3'-二甲氧基黄酮(3)、山柰酚(4)、山柰酚-3-O-β-D-吡喃葡萄糖苷(5)、山柰酚-3-O-α-L-吡喃鼠李糖苷(6)、小麦黄素(7)、小麦黄素-7-O-β-D-吡喃葡萄糖苷(8)、槲皮素(9)、槲皮素-3-O-β-D-吡喃葡萄糖苷(10)、槲皮素-3-O-α-L-吡喃鼠李糖苷(11)、草质素-3-O-β-D-吡喃葡萄糖苷(12)、草质素-7-O-β-D-吡喃葡萄糖苷(13)、草质素-7-O-α-L-吡喃鼠李糖苷(14)。结论 化合物1～3为首次从红景天属植物中分离得到,5～6、8、10～13为首次从该植物中分离得到。

Objective To study the chemical constituents from the roots and rhizomes of Rhodiola crenulata. Methods The chemical constituents were isolated by repeated silica gel chromatography and medium pressure column chromatography. Their structures were identified by various spectroscopic data including ESI-MS, ¹H-NMR, and ¹³C-NMR data. Results Fourteen compounds were isolated from the ethyl acetate fractions of R. crenulata including 3,5-dihydroxy-3',4',7-trimethoxyflavone (1), 3,5,7,3'-tetrahydroxyflavone (2), 5,4'-dihydroxy-7,3'-dimethoxyflavone (3), kaemnpferol (4), kaemnpferol-3-O-β-D-glucopyranoside (5), kaemnpferol-3-O-α-L-rhamnopyranoside (6), tricin (7), tricin-7-O-β-D-glucopyranoside (8), quercetin (9), quercetin-3-O-β-D-glucopyranoside (10), quercetin-3-O-α-L-rhamnopyranoside (11), herbacetin-3-O-β-D-glucopyranoside (12), herbacetin-7-O-β-D-glucopyranoside (13), and herbacetin-7-O-α-L-rhamnoside (14). Conclusion Compounds 1-3 are isolated from the plants in Rhodiola L. for the first time, compounds 5-6, 8, 10-13 are obtained from this plant for the first time.

科技部重大新药创制:现代中药创新集群与数字制药技术平台(2013ZX09402203)