目的 研究黄牛奶树Symplocos laurina根的三萜酸类成分。方法 采用硅胶柱色谱法、ODS、凝胶Sephadex LH-20柱色谱、半制备液相色谱等技术进行分离纯化,通过波谱数据分析及物理常数对照等方法鉴定化合物结构。结果 从黄牛奶树根95%乙醇提取物中分离得到10个三萜酸类化合物,分别鉴定为19α-羟基-4-羰基-3,24-非-2,4-开环齐墩果烷-12-烯-2,28-二酸(1)、negundonorin A(2)、2α,3β,19α,23-四羟基乌苏烷-12-烯-28-酸(3)、2α,3β,19α,23-四羟基齐墩果烷-12-烯-28-酸(4)、2α,3β,23-三羟基乌苏烷-12-烯-28-酸(5)、2α,3β,23-三羟基齐墩果烷-12-烯-28-酸(6)、2α,3α,19α,23-四羟基乌苏烷-12-烯-28-酸(7)、1α,3β,19α,23-四羟基乌苏烷-12-烯-28-酸(8)、3β,19α,23-三羟基乌苏烷-12-烯-28-酸(9)、3β,19α,23-三羟基乌苏烷-2-羰基-12-烯-28-酸(10)。结论 所有化合物均首次从黄牛奶树中分离得到,除化合物3、7,其他化合物均为首次从山矾属植物中分离得到。

Objective To study the triterpene acids from the roots of Symplocos laurina. Methods The constituents were isolated by repeated chromatography with silica gel, Sephadex LH-20, ODS columns, and semi-preparing liquid chromatography. The structures were elucidated by sepectroscopic anaylsis. Results Ten compounds were isolated from 95% ethanol extract of the roots of S. laurina and their structures were identified as: 19α-hydroxy-4-oxo-3,24-dinor-2,4-secoolean-12-en-2,28-dioic acid (1), negundonorin A (2), 2α,3β,19α,23-tetrahydroxyurs-12-en-28-oic acid (3), 2α,3β,19α,23-tetrahydroxylean-12-en-28-oic acid (4), 2α,3β,23-trihydroxyurs-12-en-28-oic acid (5), 2α,3β,23-trihydroxylean-12-en-28-oic acid (6), 2α,3α,19α,23-tetrahydroxyurs-12-en-28-oic acid (7), 1α,3β,19α,23-tetrahydroxyurs-12-en-28-oic acid (8), 3β,19α,23-trihydroxyurs-12-en-28-oic acid (9), and 2-oxo-3β,19α,23-trihydroxyurs-12-en-28-oic acid (10). Conclusion All the compounds are isolated from this plant for the first time. In addition to compounds 3 and 7, the other compounds are isolated from the plants of Symplocos Jacq for the first time.

国家自然科学基金资助项目(21103234,81373258);广东自然科学基金团队项目(S2011030003190);广州市科技计划项目(2014J4100165)