[关键词]
[摘要]
目的 利用分子印迹技术制备左旋延胡索乙素(L-THP)分子印迹聚合物(MIPs),并进行固相萃取应用研究。方法 以L-THP为模板分子,甲基丙烯酸(MAA)为功能单体,乙二醇二甲基丙烯酸酯(EDMA)为交联剂,制备左旋延胡索乙素分子印迹聚合物(L-THP-MIPs),并进行固相萃取实验优化萃取条件,考察L-THP-MIPs吸附的选择性和专一性。结果 L-THP-MIPs能特异性地吸附L-THP,而对L-THP的结构类似物延胡索甲素未表现出明显的吸附行为。结论 L-THP-MIPs对L-THP具有良好的选择性和专一性。
[Key word]
[Abstract]
Objective To prepare the molecularly imprinted polymer(MIPs) of L-tetrahydropalmatine(L-THP) by the molecular imprinting technique and study on solid-phase extraction. Methods Using L-THP as template, methyl acrylic acid(MAA) as functional monmer, and ehtylene glycol dimethacrylate(EDMA) as a cross-linking agent to prepare the L-THP-MIPs. A test was conducted to investigate the selectivity and the specificity of solid-phase extraction. Results The experiment showed that the MIPs had the specific adsorption to L-THP, but did not have the specific adsorption to corydaline the structural analogue with L-THP. Conclusion The L-THP-MIPs have a good selectivity and the specificity of L-THP.
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[基金项目]
广东省医学科学技术研究基金项目(A2015470);广东食品药品职业学院自然科学研究项目(2014YZ010)