[关键词]
[摘要]
目的 研究紫苏Perilla frutescens干燥叶的化学成分。方法 采用大孔吸附树脂柱色谱、硅胶柱色谱、ODS柱色谱和制备HPLC等技术方法进行化学成分分离,根据理化性质和波谱数据鉴定化合物结构。结果 从紫苏叶水提取物正丁醇分离部位中分离得到17个化合物,分别鉴定为(+)-isololiolide(1)、dehydrovomifoliol(2)、(-)-loliolide(3)、野黄芩苷(4)、对羟基苯甲醛(5)、对羟基苯乙酮(6)、3-吲哚甲醛(7)、反式对羟基桂皮酸(8)、芹菜素(9)、木犀草素(10)、秦皮乙素(11)、咖啡酸(12)、迷迭香酸(13)、迷迭香酸甲酯(14)、sericoside(15)、咖啡酸乙烯酯(16)、黄芩素-7-甲醚(17)。结论 化合物1~2、6~8和15为首次从紫苏属植物中分离得到。
[Key word]
[Abstract]
Objective To investigate the chemical constituents from the aqueous extract of leaves of Perilla frutescens. Methods The compounds were isolated and purified by chromatography on macroporous resin, silica gel, ODS, and preparative HPLC. Their structures were elucidated on the basis of chemical and spectroscopic methods, including MS, 1D and 2D NMR spectral techniques. Results Seventeen compounds were isolated from the aqueous extract of leaves of P. frutescens, and were identified as(+)-isololiolide(1), dehydrovomifoliol(2),(-)-loliolide(3), scutellarin(4), p-hydroxybenzaldehyde(5), p-hydroxyacetophenone(6), 3-formylindole(7), trans-p-hydroxycinnamic acid(8), apigenin(9), luteolin(10), esculetin(11), caffeic acid(12), rosmarinic acid(13), methyl rosmarinate(14), sericoside(15), caffeic acid vinyl ester(16), and negletein(17). Conclusion Compounds 1-2, 6-8, and 15 are firstly isolated from the plants of Perilla Linn.
[中图分类号]
[基金项目]
青岛大学医学院创新团队青年教师培育计划(200501)