目的 研究蛇足石杉Huperzia serrata全草的非生物碱类化学成分。方法 利用硅胶、反相硅胶、Sephadex HL-20等柱色谱进行分离纯化,通过核磁共振、质谱等谱学数据鉴定化合物结构。结果 从蛇足石杉二氯甲烷部位分离得到11个化合物,分别鉴定为千层塔烯二醇-21β-对二氢香豆酸酯(1)、千层塔烯二醇-3,21-二乙酸酯(2)、千层塔烯二醇-3-乙酸酯(3)、21-表千层塔烯二醇-3-乙酸酯(4)、3α,21β,24-千层塔烯三醇(5)、21-表千层塔烯二醇(6)、千层塔烯二醇(7)、16-羰基-3α,21β-千层塔烯二醇(8)、3β,21β,24-千层塔烯三醇(9)、16-羰基-3α,21β,24-千层塔烯三醇(10)、1-羟基二苯并呋喃醇(11)。结论 化合物1~10均为石杉型三萜类化合物,化合物11为二苯并呋喃醇类化合物;其中1为新化合物,命名为千层塔型香豆酸酯,化合物2和11首次从该植物中分离得到。

Objective To investigate the non-alkaloids in Huperzia serrata. Methods The constituents were mainly isolated and purified by means of silica gel, RP₁₈, and Sephadex LH-20 column chromatographies. The structures were identified based on analyses of the spectral data of NMR and MS. Results Totally 11 constituents were obtained from dichloromethane fraction of the plant and they were 3β-hydroxyserrat-14-en-21β-yl-p-dihydrocoumarate(1), seratenediol-3,21-diacetate(2), seratenediol-3-acetate(3), 21-epi-serratenediol-3-acetate(4), 3α,21β,24-trihydroxyserrat-14-en(5), 21-epi-serratenediol(6), serratenediol(7), 3α,21β-dihydroxy-serrat-14-en-16-one(8), 3β,21β,24-trihydroxyserrat-14-en(9), 3α,21β,24-trihydroxyserrat-14-en-16-one(10), and 1-dibenzofuranol(11). Conclusion Compounds 1-10 are serratene-type triterpenoids and compound 11 is a dibenzofuranol. Compounds 1 is a new compound named as serratcoumarate and compounds 2 and 11 are isolated from the plant for the first time.

国家自然科学基金资助项目(81261120567);上海市"科技创新行动计划"(14401901800);上海市"中药药效物质E-研究院"项目