[关键词]
[摘要]
目的 对僵蚕Bombyx Batryticatus化学成分进行分离鉴定。方法 采用溶剂提取、萃取、反复硅胶柱色谱、ODS、制备液相色谱等方法进行分离纯化,根据化合物的理化性质和光谱数据鉴定其结构。结果 从僵蚕95%乙醇提取物中分离得到12个化合物,分别鉴定为 (3α,6β)-3-苄基-6-异丙基-4-甲基-2,5-吗啡啉二酮(1)、(3α,6α)-3-苄基-6-异丙基-4-甲基-2,5-吗啡啉二酮(2)、氧麦角甾醇(3)、22E-3β-羟基-5α,6α-环氧麦角甾-22-烯-7-酮(4)、5α,6α-环氧-(22E,24R)-麦角甾-8 (14),22-二烯-3β,7α-二醇(5)、7α-甲氧基-(22E,24R)-5α,6α-环氧麦角甾-8(14),22-二烯-3β-醇(6)、(22E,24R)-麦角甾-5,7,22-三烯-3β-醇(7)、豆甾醇-7,22-二烯-3β,5α,6α-三醇(8)、3β,5α-二羟基-(22E,24R)-麦角甾-7,22-二烯-6-酮(9)、(22E,24S)-5α,8α-环二氧-24-甲基-胆甾-6,9(11),22-三烯3β-醇(10)、高精氨酸(11)、β-谷甾醇(12)。结论 化合物1~11为首次从该药材中分离得到。
[Key word]
[Abstract]
Objective To investigate the chemical constitutes of Bombyx Batryticatus. Methods The compounds were isolated by means of solvent extraction, repeated silica gel column chromatography, ODS, and preparative RP-HPLC. The structures were identified by spectral analysis and chemical evidence. Results Four compounds of steroids were isolated from the Bombyx Batryticatus and were identified as (3α,6β)-3-benzyl-6-isopropyl-4-methyl-2,5-morpholinedione (1), (3α,6α)-3-benzyl-6-isopropyl-4-methyl-2,5-morpholinedione (2), 5α,8α-epidioxyergosta-6,22-dien-3β-ol (3), 22E-3β-hydroxy-5α,6α-epoxyergosta-22-en-7-one (4), 5α,6α-epoxy-(22E,24R)-ergosta-8(14),22-diene-3β,7α-diol (5), 7α-methoxy-(22E,24R)-5α,6α-epoxyergosta-8(14),22-diene-3β-ol (6), (22E,24R)-ergosta-5,7,22-trien-3β-ol (7), stigmasta-7,22-diene-3β,5α,6α-triol (8), 3β,5α-dihydroxy-(22E,24R)-ergosta-7,22-diene-6-one (9), (22E,24S)-5α,8α-epidioxy-24-methylcholesta-6,9(11),22-trien-3β-ol (10), homoarginine (11), and β-sitosterol (12). Conclusion Compounds 1-11 are isolated from Bombyx Batryticatus for the first time.
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[基金项目]
国家科技支撑计划2012(BAI30B02)