[关键词]
[摘要]
目的 对福建金线莲Anoectochilus roxburghii、台湾金线莲A. formosanus和滇越金线莲A. chapaensis进行生药学鉴别。方法 采用徒手切片、显微观察和数码照相方法,比较分析不同植物基原金线莲的植物形态特征、根、茎、叶的横切面组织构造及其组织特征,金线莲干燥药材粉末特征。结果 福建金线莲和台湾金线莲较滇越金线莲植株小,三者叶脉颜色分别为金黄色、银白色和淡红色,是外观鉴别的依据之一;滇越金线莲根木质部束数量较多,且髓部宽广,茎外韧型维管束数量较多;上表皮细胞形状、中脉上下表面形状与细胞色素分布特点是叶横切面的鉴别点;台湾金线莲上表皮细胞形状为类三角形,其他2种植物为类椭圆形;滇越金线莲中脉上表面较平整,其他2种植物微凹;福建金线莲中脉下表面呈半圆形凸出,其他2种植物微凸,福建金线莲红色物质稀少且位于上表面,台湾金线莲红色物质较多且散在,滇越金线莲有红色物质位于上表皮且集中排一列。结论 不同植物基原金线莲植物形态和显微存在差异,为建立金线莲质量标准提供依据。
[Key word]
[Abstract]
Objective To research the pharmaceutical identification of Anoectochilus roxburghii (Wall.) Lindl., A. formosanus Hayata and A. chapaensis Gagnep. Methods Using the methods of freehand section, microexamination, and digital photography to compare the plant morphology, tissue structures of root, stem and leaf transverse, and dried medicinal powder. Results The plants of A.roxburghii and A. formosanus were smaller than that of A. chapaensis. The colors of their leaf veins were golden yellow, silver and light red, respectively, which should be the key morphologic identification. As for characteristics of histology, there were more xylem bundles and broad pulp in the root, and more external vascular bundles in the stem of A. chapaensis than the other two plants. The upper epidermal cells of A. formosanus were triangle shape differ to the oval shape in the other two plants. There were few red materials on the upper surface of A. roxburghii. Many red materials on the upper surface of A. formosanus were dispersed existence. The red materials on the upper surface of A. chapaensis showed to concentrate a column. The result showed their chemical compositions were large differences. Conclusion The large differences on plant morphology and microstructure of the different sources plant were carried out. Our works will provide the basis for the establishment of quality standard of Anoectochilus.
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[基金项目]
福州市科学技术局市校(院所)科技合作项目(2014-G-61);福建中医药大学-重点学科专项(X2014138-学科)