目的 检测内生拮抗菌JYg07在五味子Schisandra chinensis植株内的定殖情况.方法 利用抗生素标记法获得JYg07菌株的抗链霉素和利福平的双抗突变体菌株,采用灌根、喷雾、注射和刺伤叶片等方法接种,测定JYg07菌株在五味子植株内的定殖情况.结果 JYg07菌株可以通过自然孔口和伤口进入五味子体内,并能够在土壤中长期存活;菌液浓度主要影响定殖高峰到来的时间;4种接种方式均可以使JYg07菌株在五味子体内稳定的定殖并传导,并且在根中的定殖能力要高于茎和叶.高浓度接种培养液、灌根接种的方式可以保证JYg07菌株在五味子植株中达到最佳定殖状态.结论 JYg07菌株可以在五味子体内稳定定殖并传导,能够作为新的生防菌资源开发利用.

Objective The colonization of endophytic antagonistic bacteria in different parts of Schisandra chinensis was tested in the meantime. Methods Using antibiotic notation method, mutant strains of JYg07 resistant to streptomycin and rifampin at the same time were obtained. To test the colonization the labeled strains of JYg07 with different vaccination methods of root irrigation, spray, injection, and stabbing leaves. Results Through the natural orifice and the wound, the strain JYg07 could enter the plant of S. chinensis and long live in soil. Bacterial liquid concentration influenced the time of colonization peak. Using four vaccination methods, the labeled strains of JYg07 could colonize and engraft rapidly in S. chinensis, and the colonization ability in the roots was higher than that in the stems and leaves. High concentration of nutrient solution method and root irrigation method could make JYg07 in the best colonization state in S. chinensis. Conclusion Strain JYg07 could colonize and engraft rapidly in S. chinensis, which could be developedt and utilized as new resources of biocontrol bacteria.

吉林省世行办质量安全项目(2011-Z24)