目的 考察不同因素对罗汉果止咳作用的影响。方法 采用氨水引咳法,按序贯法(即上下法)求半数有效量的原理,计算小鼠半数引咳时间(EDT₅₀),以给药组与对照组EDT₅₀比值(R)评价不同产地、育苗方式、果实生长期、商品规格罗汉果以及干罗汉果与鲜罗汉果的止咳作用。结果 湖南衡阳、广西南宁、广西永福县龙江及百寿采收的罗汉果均有止咳作用(R>130%),尤其是广西永福县龙江和百寿的罗汉果止咳作用显著(R>150%);扦插苗和组培苗生长得到的罗汉果均有止咳作用(R>130%);果实生长期40～45 d和60～70 d的未成熟果无止咳作用(R<130%);鲜果和干果都有止咳作用(R>130%),但干果作用显著(R>150%);一级果和响果有止咳作用(R>130%),二级果没有止咳作用(R<130%)。结论 不同产地、繁育技术、是否烘干对罗汉果的止咳作用影响不大,但果实生长期和商品规格对罗汉果的止咳作用有影响。

Objective To observe the effects of various factors on the antitussive effect of Siraitia grosvenorii. Methods According to the sequential method (i.e., up and down method) for the principle of median effective dose, the antitussive effect of S. grosvenorii was observed and the median effective time (EDT₅₀) of cough induced by NH₃ aq. in mice was calculated. Results S. grosvenorii from Hunan-Hengyang, Nanning-Tanluo, Yongfu-Longjiang, and Baishou have the antitussive effect, especially in Yongfu-Longjiang and Baishou (R > 150%). The fruits obtained from cuttage seedlings and tissue culture seedlings have the antitussive effect, while the immature fruits have not (R < 130%). Both the fresh fruit and dried fruit (R > 130%) have the significant antitussive effect, and so first class fruit and ringing fruit do (R > 130%). However secondary class fruit does not have the antitussive effect (R < 130%). Conclusion The factors, except the habitat, breeding technology, and whether drying or not, such as growth period and commercial specification may influence the antitussive effect of S. grosvenori.

广西自然科学基金资助项目(2012GXNSFBA053088);广西教育厅科研重点课题(201202ZD054);国家自然科学基金资助项目(81160518)