目的 比较川麦冬与杭麦冬中3种高异黄酮类活性成分的质量分数差异及麦冬水提物的抗氧化能力的差异。方法 采用超高效液相色谱与二极管阵列检测器技术,测定3种高异黄酮类活性成分。色谱柱为ACQUITY^TM UPLC BEH C₁₈色谱柱(100 mm×2.1 mm,1.7 μm),流动相为乙腈-0.2%甲酸水(55:45),体积流量0.2 mL/min,检测波长296 nm。采用紫外分光光度法测定麦冬水提物清除DPPH自由基的活性。结果 川麦冬中甲基麦冬高异黄酮A、甲基麦冬黄烷酮A与甲基麦冬黄烷酮B的平均质量分数分别为(3.06±0.54)、(40.10±5.63)、(29.51±5.06)μg/g;杭麦冬中3种成分平均质量分数分别为(9.22±3.52)、(106.63±27.56)、(256.97±61.79)μg/g。川麦冬水提物清除自由基的IC₅₀平均值为16.59 mg/mL,杭麦冬的IC₅₀平均值为14.48 mg/mL,阳性对照VC的IC₅₀值为7.06 μg/mL。结论 与川麦冬比较,杭麦冬中高异黄酮类成分的量较高,其水提取物对DPPH自由基的清除作用也较强,说明杭麦冬具有较强的抗氧化活性,为麦冬的质量评价及其道地性研究提供依据。

Objective To compare the contents of three homoisoflavones and their anti-oxidative activity of Ophiopogon japonicus in Sichuan (OJS) and Zhejiang (OJZ). Methods The determination was performed on ACQUITY^TM UPLC BEH C₁₈ column (100 mm × 2.1 mm, 1.7 μm), the Ultra Performance Liquid Chromatography-Photo-Diode Array (UPLC-PDA) technology was applied, and the mobile phase consisted of acetonitrile and 0.2% formic acid aqueous solution (55 : 45) with gradient elution program, flow rate was 0.20 mL/min with 2 μL of sample quantity at 296 nm, and the anti-DPPH radical efficiency of water extract from Ophiopogonis Radix were evaluated by UV-photometer. Results The average values of methylophiopogonone A, methylophiopogonanone A, and methylophiopogonanone B were (3.06±0.54), (40.10±5.63), and (29.51±5.06) μg/g in OJS, respectively; while those in OJZ were (9.22±3.52), (106.63±27.56), and (256.97±61.79) μg/g, separately. The IC₅₀ value was 16.59 mg/mL in OJS, while that in OJZ was 14.48 mg/mL. The IC₅₀ value of positive control VC was 7.06 μg/mL. Conclution Compared with OJS, the contents of homoisoflavones in OJZ are higher, and the anti-radical efficiency of water extract from OJZ is stronger. It provides the basis for the quality evaluation and geo-authentic research of Ophiopogonis Radix.

国家自然科学基金资助项目(81130069)